Rab 5 Antibody (D-11): sc-46692

Rab 5 Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rab 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rab 5 (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 5 protein plays a crucial role in regulating endocytosis and protein transport within cells, particularly in moving carrier vesicles from endoplasmic reticulum to Golgi apparatus and ultimately to secretory vesicles. This process maintains cellular homeostasis and facilitates communication between different cellular compartments. Rab 5 interacts with various effector proteins essential for vesicle docking and fusion, ensuring proteins are delivered to correct destinations. Proper functioning of Rab 5 is critical, as disruptions in activity can lead to various diseases, including neurodegenerative disorders and cancer, highlighting studying importance in both basic and clinical research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rab 5 Antibody (D-11) References:

1. GTP-binding proteins in intracellular transport.  |  Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
2. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach.  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
3. Cloning of a Rab3 isotype predominantly expressed in adipocytes.  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
4. Rab 5 is required for the cellular entry of dengue and West Nile viruses.  |  Krishnan, MN., et al. 2007. J Virol. 81: 4881-5. PMID: 17301152
5. RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.  |  Smurova, K. and Podbilewicz, B. 2016. Cell Rep. 14: 1517-1527. PMID: 26854231
6. LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling.  |  Liu, H., et al. 2018. J Cell Biol. 217: 299-314. PMID: 29079669
7. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
8. Coatomers and SNAREs in promoting membrane traffic.  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
9. Friends and family: the role of the Rab GTPases in vesicular traffic.  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
10. The role of GTP-binding proteins in transport along the exocytic pathway.  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
11. Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation.  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
12. Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins.  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rab 5 Antibody (D-11) has 112 citations in a variety of scientific publications.

Primary antibodies like Anti-Rab 5 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rab 5 Antibody (D-11) Specifications

Alternate Product Name: RAB5

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-215

Epitope Target: Rab 5A

Epitope Species: human

Product Reactivity/Detection: Rab 5A, 5B and 5C; partially cross reactive with other Rab family members

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 112 citations in various scientific publications

Available Conjugates/Forms for Rab 5 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin