Rab 32 Antibody (B-4): sc-390178
- Rab 32 Antibody (B-4) is a mouse monoclonal IgG1 κ Rab 32 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 181-223 mapping at the C-terminus of Rab 32 of mouse origin
- Rab 32 Antibody (B-4) is recommended for detection of Rab 32 of mouse origin by WB, IP, IF and ELISA
- Anti-Rab 32 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 32 Antibody (B-4) for WB applications. These reagents are now offered in bundles with Rab 32 Antibody (B-4) (see ordering information below).
Rab 32 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rab 32 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rab 32 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 32, consisting of 225 amino acids, is a member of the small GTPase superfamily and plays a crucial role in vesicle and granule targeting within cells. Notably, Rab 32 has a unique GTP-binding sequence, DIAGQE, which differs from the more common DTAGQE found in other RAB proteins, highlighting distinct functional properties. Rab 32 acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A, effectively anchoring it to mitochondria, which is vital for regulating mitochondrial dynamics and function. Rab 32 is widely expressed in various tissues, including heart, liver, kidney, bone marrow, testis, colon, and fetal lung, and has been implicated in the pathogenesis of certain cancers, such as microsatellite instability-high colon cancers, where Rab 32 is frequently hypermethylated. The gene encoding Rab 32 is conserved across multiple species, including chimpanzees, cows, mice, rats, chickens, zebrafish, fruit flies, mosquitoes, and Caenorhabditis elegans, and is located on human chromosome 6q24.3, underscoring evolutionary significance and potential as a biomarker in cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rab 32 Antibody (B-4) References:
- Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins. | Herrera-Cruz, MS., et al. 2021. Biol Direct. 16: 22. PMID: 34743744
- Rab32/38-Dependent and -Independent Transport of Tyrosinase to Melanosomes in B16-F1 Melanoma Cells. | Nishizawa, A., et al. 2022. Int J Mol Sci. 23: PMID: 36430618
- Characterization of Rab32- and Rab38-positive lysosome-related organelles in osteoclasts and macrophages. | Noda, K., et al. 2023. J Biol Chem. 299: 105191. PMID: 37625588
- Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclasts. | Tokuda, K., et al. 2023. Cell Struct Funct. 48: 223-239. PMID: 37793839
- Rab32 family proteins regulate autophagosomal components recycling. | Wu, Z., et al. 2024. J Cell Biol. 223: PMID: 38323995
- Deciphering the molecular regulatory of RAB32/GPRC5A axis in chronic obstructive pulmonary disease. | Wu, Y., et al. 2024. Respir Res. 25: 116. PMID: 38448858
- LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis. | Li, J., et al. 2024. J Cell Biol. 223: PMID: 39078368
- RAB32 mutation in Parkinson's disease. | Monfrini, E., et al. 2024. Lancet Neurol. 23: 961-962. PMID: 39304252
- RAB32 mutation in Parkinson's disease. | Zhao, Y., et al. 2024. Lancet Neurol. 23: 962-963. PMID: 39304253
- Genetic and Epidemiological Insights into RAB32-Linked Parkinson's Disease. | Radefeldt, M., et al. 2025. Mov Disord. 40: 147-151. PMID: 39460989
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 32 Antibody (B-4) | sc-390178 | 200 µg/ml | $316.00 | |||
Rab 32 Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-530085 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rab 32 Antibody (B-4): m-IgGκ BP-HRP Bundle | sc-523436 | 200 µg Ab, 40 µg BP | $354.00 | |||
Rab 32 Antibody (B-4): m-IgG1 BP-HRP Bundle | sc-543861 | 200 µg Ab; 20 µg BP | $354.00 | |||
Rab 32 Antibody (B-4) AC | sc-390178 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rab 32 Antibody (B-4) HRP | sc-390178 HRP | 200 µg/ml | $316.00 | |||
Rab 32 Antibody (B-4) FITC | sc-390178 FITC | 200 µg/ml | $330.00 | |||
Rab 32 Antibody (B-4) PE | sc-390178 PE | 200 µg/ml | $343.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 488 | sc-390178 AF488 | 200 µg/ml | $357.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 546 | sc-390178 AF546 | 200 µg/ml | $357.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 594 | sc-390178 AF594 | 200 µg/ml | $357.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 647 | sc-390178 AF647 | 200 µg/ml | $357.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 680 | sc-390178 AF680 | 200 µg/ml | $357.00 | |||
Rab 32 Antibody (B-4) Alexa Fluor® 790 | sc-390178 AF790 | 200 µg/ml | $357.00 |