Rab 32 Antibody (D-11): sc-377472

Rab 32 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rab 32 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 32 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 32 is a 225 amino acid protein that belongs to the small GTPase superfamily and plays a crucial role in vesicle and granule targeting within cells. Notably, Rab 32 has an atypical GTP-binding sequence, DIAGQE, which differs from the more common DTAGQE found in other RAB proteins, highlighting unique functional properties. Rab 32 acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A, effectively anchoring it to mitochondria, which is vital for regulating mitochondrial dynamics and function. Rab 32 is widely expressed in various tissues, including heart, liver, kidney, bone marrow, testis, colon, and fetal lung, and has been implicated in the pathogenesis of certain cancers, such as microsatellite instability-high colon cancers, where Rab 32 is frequently hypermethylated. The gene encoding Rab 32 is conserved across multiple species, including chimpanzees, bovines, mice, rats, chickens, zebrafish, fruit flies, mosquitoes, and Caenorhabditis elegans, and is located on human chromosome 6q24.3, underscoring evolutionary significance and potential as a biomarker in cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rab 32 Antibody (D-11) References:

1. Molecular cloning, bacterial expression and properties of Rab31 and Rab32.  |  Bao, X., et al. 2002. Eur J Biochem. 269: 259-71. PMID: 11784320
2. Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics.  |  Alto, NM., et al. 2002. J Cell Biol. 158: 659-68. PMID: 12186851
3. Structural determinants of Rab and Rab Escort Protein interaction: Rab family motifs define a conserved binding surface.  |  Pereira-Leal, JB., et al. 2003. Biochem Biophys Res Commun. 301: 92-7. PMID: 12535645
4. Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein kinase A anchoring.  |  Alto, NM., et al. 2003. Proc Natl Acad Sci U S A. 100: 4445-50. PMID: 12672969
5. The DNA sequence and analysis of human chromosome 6.  |  Mungall, AJ., et al. 2003. Nature. 425: 805-11. PMID: 14574404
6. RAB32 hypermethylation and microsatellite instability in gastric and endometrial adenocarcinomas.  |  Shibata, D., et al. 2006. Int J Cancer. 119: 801-6. PMID: 16557577
7. A small GTPase, human Rab32, is required for the formation of autophagic vacuoles under basal conditions.  |  Hirota, Y. and Tanaka, Y. 2009. Cell Mol Life Sci. 66: 2913-32. PMID: 19593531
8. Rab32 restriction of intracellular bacterial pathogens.  |  Solano-Collado, V., et al. 2018. Small GTPases. 9: 216-223. PMID: 27645564
9. Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.  |  Chen, P., et al. 2023. Cell Death Dis. 14: 198. PMID: 36922509
10. Rab32 facilitates Schwann cell pyroptosis in rats following peripheral nerve injury by elevating ROS levels.  |  Wang, J., et al. 2024. J Transl Med. 22: 194. PMID: 38388913
11. RAB32 Ser71Arg in autosomal dominant Parkinson's disease: linkage, association, and functional analyses.  |  Gustavsson, EK., et al. 2024. Lancet Neurol. 23: 603-614. PMID: 38614108
12. Systematic rare variant analyses identify RAB32 as a susceptibility gene for familial Parkinson's disease.  |  Hop, PJ., et al. 2024. Nat Genet. 56: 1371-1376. PMID: 38858457

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rab 32 Antibody (D-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Rab 32 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rab 32 Antibody (D-11) Specifications

Alternate Product Name: Rab 32

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-225

Epitope Target: Rab 32

Epitope Species: human

Product Reactivity/Detection: Rab 32

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Rab 32 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin