Rab 32 Antibody (B-11): sc-390206

Rab 32 Antibody (B-11) is a mouse monoclonal IgG2a antibody that detects Rab 32 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rab 32 is a 225 amino acid protein that belongs to the small GTPase superfamily and plays a crucial role in vesicle and granule targeting, particularly in mitochondrial dynamics. Notably, Rab 32 has an atypical GTP-binding sequence, DIAGQE, which distinguishes it from other members of the Rab family that typically possess the DTAGQE sequence. This unique feature may influence Rab 32′s interaction with downstream effectors and regulatory mechanisms. Additionally, Rab 32 functions as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A, effectively anchoring it to mitochondria, which is vital for maintaining mitochondrial function and energy metabolism. Rab 32 is widely expressed, with particularly high levels found in the heart, liver, kidney, bone marrow, testis, colon, and fetal lung, underscoring its importance in various physiological processes. Furthermore, Rab 32 has been implicated in cancer biology, as Rab 32 is frequently hypermethylated in microsatellite instability-high colon cancers, and Rab 32 methylation status is associated with hMLH1 hypermethylation in gastric adenocarcinomas. The conservation of Rab 32 across multiple species, including chimpanzees, cows, mice, rats, chickens, zebrafish, fruit flies, mosquitoes, and C. elegans, highlights its fundamental biological role, with Rab 32 mapping to human chromosome 6q24.3. Rab 32 antibody (B-11) is an essential tool for researchers investigating the intricate roles of this protein in cellular processes and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rab 32 Antibody (B-11) References:

1. RAB32 hypermethylation and microsatellite instability in gastric and endometrial adenocarcinomas.  |  Shibata, D., et al. 2006. Int J Cancer. 119: 801-6. PMID: 16557577
2. Cell type-specific Rab32 and Rab38 cooperate with the ubiquitous lysosome biogenesis machinery to synthesize specialized lysosome-related organelles.  |  Bultema, JJ. and Di Pietro, SM. 2013. Small GTPases. 4: 16-21. PMID: 23247405
3. RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes.  |  Marubashi, S., et al. 2016. J Biol Chem. 291: 1427-40. PMID: 26620560
4. Hepatitis C Virus-Induced Rab32 Aggregation and Its Implications for Virion Assembly.  |  Pham, TM., et al. 2017. J Virol. 91: PMID: 27852857
5. Rab32-related antimicrobial pathway is involved in the progression of dextran sodium sulfate-induced colitis.  |  Xie, X., et al. 2018. FEBS Open Bio. 8: 1658-1668. PMID: 30338217
6. Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking.  |  Waschbüsch, D., et al. 2019. PLoS One. 14: e0208889. PMID: 30640902
7. LRRK2 binds to the Rab32 subfamily in a GTP-dependent manner via its armadillo domain.  |  McGrath, E., et al. 2021. Small GTPases. 12: 133-146. PMID: 31552791
8. The small GTPase Rab32 resides on lysosomes to regulate mTORC1 signaling.  |  Drizyte-Miller, K., et al. 2020. J Cell Sci. 133: PMID: 32295849
9. Proteolysis of Rab32 by Salmonella GtgE induces an inactive GTPase conformation.  |  Savitskiy, S., et al. 2021. iScience. 24: 101940. PMID: 33426511
10. High Expression of RAB32 Predicts Adverse Outcomes: A Potential Therapeutic Target for Glioblastoma.  |  Huang, L., et al. 2024. J Cancer. 15: 6710-6723. PMID: 39668831

The preferred Rab 32 antibody is Rab 32 Antibody (D-11): sc-377472.