Rab 3 GAP p150 Antibody (H-7): sc-398314
- Rab 3 GAP p150 Antibody (H-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 64-173 mapping near the N-terminus of Rab 3 GAP p150 of human origin
- Anti-Rab 3 GAP p150 Antibody (H-7) is recommended for detection of Rab 3 GAP p150 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
- Anti-Rab 3 GAP p150 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 3 GAP p150 Antibody (H-7) for WB applications. These reagents are now offered in bundles with Rab 3 GAP p150 Antibody (H-7) (see ordering information below).
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Rab 3 GAP p150 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rab 3 GAP p150 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 3 GAP p150 antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 3 GAP p150, also referred to as RAB3GAP2 or the Rab3 GTPase-activating protein non-catalytic subunit, is a crucial protein consisting of 1,393 amino acids and is part of the Rab3-GAP regulatory subunit family. This protein plays a significant role in regulating synaptic vesicle exocytosis, which is essential for neurotransmitter release and proper neuronal communication. Defects in Rab 3 GAP p150 are linked to Martsolf and Warburg Micro syndrome, both of which present with congenital cataracts, microphthalmia, postnatal microcephaly, and developmental delays, inherited in an autosomal recessive manner. The Rab3 GTPase-activating complex, which includes Rab 3 GAP p150, forms a heterodimer with RAB3GAP and interacts with DMXL2, highlighting its importance in cellular signaling pathways. The gene encoding Rab 3 GAP p150 is conserved across various species, including chimpanzees, dogs, cows, rats, chickens, zebrafish, fruit flies, mosquitoes, Arabidopsis thaliana, and rice, and maps to human chromosome 1q41.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rab 3 GAP p150 Antibody (H-7) References:
- Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1998. DNA Res. 5: 355-64. PMID: 10048485
- Mutations of the catalytic subunit of RAB3GAP cause Warburg Micro syndrome. | Aligianis, IA., et al. 2005. Nat Genet. 37: 221-3. PMID: 15696165
- Mutation in Rab3 GTPase-activating protein (RAB3GAP) noncatalytic subunit in a kindred with Martsolf syndrome. | Aligianis, IA., et al. 2006. Am J Hum Genet. 78: 702-7. PMID: 16532399
- The DNA sequence and biological annotation of human chromosome 1. | Gregory, SG., et al. 2006. Nature. 441: 315-21. PMID: 16710414
- Rab GTPases and their roles in brain neurons and glia. | Ng, EL. and Tang, BL. 2008. Brain Res Rev. 58: 236-46. PMID: 18485483
- KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through DENN/MADD. | Niwa, S., et al. 2008. Nat Cell Biol. 10: 1269-79. PMID: 18849981
- Molecular cloning and characterization of the noncatalytic subunit of the Rab3 subfamily-specific GTPase-activating protein. | Nagano, F., et al. 1998. J Biol Chem. 273: 24781-5. PMID: 9733780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 3 GAP p150 Antibody (H-7) | sc-398314 | 200 µg/ml | $322.00 | |||
Rab 3 GAP p150 Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-527763 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rab 3 GAP p150 Antibody (H-7): m-IgG1 BP-HRP Bundle | sc-533136 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rab 3 GAP p150 Antibody (H-7) AC | sc-398314 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rab 3 GAP p150 Antibody (H-7) HRP | sc-398314 HRP | 200 µg/ml | $322.00 | |||
Rab 3 GAP p150 Antibody (H-7) FITC | sc-398314 FITC | 200 µg/ml | $336.00 | |||
Rab 3 GAP p150 Antibody (H-7) PE | sc-398314 PE | 200 µg/ml | $349.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 488 | sc-398314 AF488 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 546 | sc-398314 AF546 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 594 | sc-398314 AF594 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 647 | sc-398314 AF647 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 680 | sc-398314 AF680 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p150 Antibody (H-7) Alexa Fluor® 790 | sc-398314 AF790 | 200 µg/ml | $364.00 |