R-cadherin Antibody (D-9): sc-398306

R-cadherin Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects R-cadherin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). R-cadherin Antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. R-cadherin, also known as cadherin-4, is a member of the cadherin family of calcium-dependent adhesion molecules, which play a crucial role in mediating cell-cell adhesion essential for maintaining tissue integrity and facilitating morphogenetic processes. R-cadherin is predominantly located in the cell membrane, where R-cadherin forms adherens junctions that connect adjacent cells, thereby contributing to the structural stability of tissues. This localization is particularly important in the retina, where R-cadherin is involved in the development and maintenance of retinal architecture, as well as in the formation of striated muscle and epithelial tissues. The extracellular domain of R-cadherin contains five homologous repeats that are critical for binding specificity, while the intracellular domain interacts with cytoplasmic proteins, such as catenin beta, to regulate adhesive functions. The ability of R-cadherin to mediate these interactions underscores R-cadherin′s significance in developmental biology and tissue organization, making anti-R-cadherin antibody (D-9) an invaluable tool for researchers studying cell adhesion and tissue dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

R-cadherin Antibody (D-9) References:

1. Retinal vascular development is mediated by endothelial filopodia, a preexisting astrocytic template and specific R-cadherin adhesion.  |  Dorrell, MI., et al. 2002. Invest Ophthalmol Vis Sci. 43: 3500-10. PMID: 12407162
2. Adult bone marrow-derived stem cells use R-cadherin to target sites of neovascularization in the developing retina.  |  Dorrell, MI., et al. 2004. Blood. 103: 3420-7. PMID: 14726407
3. Genomic organization and chromosomal mapping of the mouse P-cadherin gene.  |  Hatta, M., et al. 1991. Nucleic Acids Res. 19: 4437-41. PMID: 1886768
4. Dental pulp cells derived from permanent teeth express higher levels of R-cadherin than do deciduous teeth: implications of the correlation between R-cadherin expression and restriction of multipotency in mesenchymal stem cells.  |  Takahashi, N., et al. 2012. Arch Oral Biol. 57: 44-51. PMID: 21851926
5. Long non-coding RNA linc-cdh4-2 inhibits the migration and invasion of HCC cells by targeting R-cadherin pathway.  |  Gao, Y., et al. 2016. Biochem Biophys Res Commun. 480: 348-354. PMID: 27765630
6. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.  |  Takeichi, M. 1988. Development. 102: 639-55. PMID: 3048970
7. Desmosomal cadherins: another growing multigene family of adhesion molecules.  |  Koch, PJ. and Franke, WW. 1994. Curr Opin Cell Biol. 6: 682-7. PMID: 7833048
8. Spatial and temporal relationships between cadherins and PECAM-1 in cell-cell junctions of human endothelial cells.  |  Ayalon, O., et al. 1994. J Cell Biol. 126: 247-58. PMID: 8027182
9. Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly.  |  Hinck, L., et al. 1994. J Cell Biol. 125: 1327-40. PMID: 8207061
10. Morphogenetic roles of classic cadherins.  |  Takeichi, M. 1995. Curr Opin Cell Biol. 7: 619-27. PMID: 8573335
11. A potential role of R-cadherin in striated muscle formation.  |  Rosenberg, P., et al. 1997. Dev Biol. 187: 55-70. PMID: 9224674
12. Cadherins and catenins: interactions and functions in embryonic development.  |  Ranscht, B. 1994. Curr Opin Cell Biol. 6: 740-6. PMID: 7833053

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-R-cadherin Antibody (D-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-R-cadherin Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

R-cadherin Antibody (D-9) Specifications

Alternate Product Name: CDH4

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 190-223

Epitope Target: R-cadherin

Epitope Species: human

Product Reactivity/Detection: R-cadherin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for R-cadherin Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin