QSK Antibody (C-2): sc-515408
- QSK Antibody (C-2) is a mouse monoclonal IgG2b κ QSK antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 460-616 mapping within an internal region of QSK of human origin
- QSK Antibody (C-2) is recommended for detection of QSK of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-QSK Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for QSK Antibody (C-2) for WB applications. These reagents are now offered in bundles with QSK Antibody (C-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
QSK Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects QSK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). QSK (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. QSK protein, also known as L19 or SIK3 (salt-inducible kinase 3), plays a crucial role in cellular signaling pathways, particularly in regulating metabolic processes and stress responses. QSK (C-2) monoclonal antibody recognizes a 1,263 amino acid cytoplasmic protein that belongs to the serine/threonine protein kinase family and is characterized by a unique structure, including a protein kinase domain and a ubiquitin-associated (UBA) domain. These domains facilitate QSK′s interaction with various substrates and regulatory proteins, thereby influencing key cellular functions such as steroid hormone biosynthesis and insulin signaling in adipocytes. QSK is activated by 14-3-3 ζ and requires magnesium as a cofactor for enzymatic activity, highlighting the importance of metal ions in function. Additionally, QSK exists in three alternatively spliced isoforms, which may contribute to functional diversity and regulatory mechanisms within different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
QSK Antibody (C-2) References:
- SIK (Salt-inducible kinase): regulation of ACTH-mediated steroidogenic gene expression and nuclear/cytosol redistribution. | Lin, X., et al. 2000. Endocr Res. 26: 995-1002. PMID: 11196481
- Salt-inducible kinase is involved in the ACTH/cAMP-dependent protein kinase signaling in Y1 mouse adrenocortical tumor cells. | Lin, X., et al. 2001. Mol Endocrinol. 15: 1264-76. PMID: 11463852
- Salt-inducible kinase represses cAMP-dependent protein kinase-mediated activation of human cholesterol side chain cleavage cytochrome P450 promoter through the CREB basic leucine zipper domain. | Doi, J., et al. 2002. J Biol Chem. 277: 15629-37. PMID: 11864972
- Salt-inducible kinase-mediated regulation of steroidogenesis at the early stage of ACTH-stimulation. | Takemori, H., et al. 2003. J Steroid Biochem Mol Biol. 85: 397-400. PMID: 12943728
- Salt-inducible kinase in steroidogenesis and adipogenesis. | Okamoto, M., et al. 2004. Trends Endocrinol Metab. 15: 21-6. PMID: 14693422
- Salt-inducible kinase (SIK) isoforms: their involvement in steroidogenesis and adipogenesis. | Katoh, Y., et al. 2004. Mol Cell Endocrinol. 217: 109-12. PMID: 15134808
- Importance of autophosphorylation at Ser186 in the A-loop of salt inducible kinase 1 for its sustained kinase activity. | Hashimoto, YK., et al. 2008. J Cell Biochem. 104: 1724-39. PMID: 18348280
- Intracellular sodium sensing: SIK1 network, hormone action and high blood pressure. | Jaitovich, A. and Bertorello, AM. 2010. Biochim Biophys Acta. 1802: 1140-9. PMID: 20347966
- Downregulation of SIK2 expression promotes the melanogenic program in mice. | Horike, N., et al. 2010. Pigment Cell Melanoma Res. 23: 809-19. PMID: 20819186
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QSK Antibody (C-2) | sc-515408 | 200 µg/ml | $322.00 | |||
QSK Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-531356 | 200 µg Ab; 10 µg BP | $361.00 | |||
QSK Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-525027 | 200 µg Ab, 40 µg BP | $361.00 | |||
QSK Antibody (C-2): m-IgG2b BP-HRP Bundle | sc-550281 | 200 µg Ab; 10 µg BP | $361.00 | |||
QSK Antibody (C-2) AC | sc-515408 AC | 500 µg/ml, 25% agarose | $424.00 | |||
QSK Antibody (C-2) HRP | sc-515408 HRP | 200 µg/ml | $322.00 | |||
QSK Antibody (C-2) FITC | sc-515408 FITC | 200 µg/ml | $336.00 | |||
QSK Antibody (C-2) PE | sc-515408 PE | 200 µg/ml | $349.00 | |||
QSK Antibody (C-2) Alexa Fluor® 488 | sc-515408 AF488 | 200 µg/ml | $364.00 | |||
QSK Antibody (C-2) Alexa Fluor® 546 | sc-515408 AF546 | 200 µg/ml | $364.00 | |||
QSK Antibody (C-2) Alexa Fluor® 594 | sc-515408 AF594 | 200 µg/ml | $364.00 | |||
QSK Antibody (C-2) Alexa Fluor® 647 | sc-515408 AF647 | 200 µg/ml | $364.00 | |||
QSK Antibody (C-2) Alexa Fluor® 680 | sc-515408 AF680 | 200 µg/ml | $364.00 | |||
QSK Antibody (C-2) Alexa Fluor® 790 | sc-515408 AF790 | 200 µg/ml | $364.00 |