PWP1 Antibody (B-2): sc-166656
- PWP1 Antibody (B-2) is a mouse monoclonal IgG2a κ PWP1 antibody provided at 200 µg/ml
- raised against amino acids 143-221 mapping within an internal region of PWP1 of human origin
- PWP1 Antibody (B-2) is recommended for detection of PWP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PWP1 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PWP1 Antibody (B-2) for WB applications. These reagents are now offered in bundles with PWP1 Antibody (B-2) (see ordering information below).
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PWP1 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects PWP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PWP1 Antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PWP1, or Periodic Tryptophan Protein 1, is a 501 amino acid protein that serves as the human homolog of the Saccharomyces cerevisiae periodic Trp protein. PWP1 is primarily localized in the nucleus and is highly expressed in the kidneys, pancreas, placenta, and skeletal muscle, where PWP1 plays a crucial role in transcription regulation and cell growth. Notably, PWP1 is involved in the interaction with chromatin through association with histone tails, particularly the H4 tail, which is essential for maintaining chromatin structure and function. The presence of five WD-repeats in PWP1 suggests potential involvement in protein-protein interactions, which may contribute to the development of pancreatic cancer and the regulation of chaperone activities in the endoplasmic reticulum, as well as signal transduction pathways in the nucleus.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PWP1 Antibody (B-2) References:
- Endonuclein is a cell cycle regulated WD-repeat protein that is up-regulated in adenocarcinoma of the pancreas. | Honoré, B., et al. 2002. Oncogene. 21: 1123-9. PMID: 11850830
- The functional landscape of mouse gene expression. | Zhang, W., et al. 2004. J Biol. 3: 21. PMID: 15588312
- Comparative analysis of the beta transducin family with identification of several new members including PWP1, a nonessential gene of Saccharomyces cerevisiae that is divergently transcribed from NMT1. | Duronio, RJ., et al. 1992. Proteins. 13: 41-56. PMID: 1594577
- The WD40-repeat protein Pwp1p associates in vivo with 25S ribosomal chromatin in a histone H4 tail-dependent manner. | Suka, N., et al. 2006. Nucleic Acids Res. 34: 3555-67. PMID: 16855292
- Nuclear protein profiling of Jurkat cells during heat stress-induced apoptosis by 2-DE and MS/MS. | Yuan, X., et al. 2007. Electrophoresis. 28: 2018-26. PMID: 17523140
- Cloning of a cDNA encoding a novel human nuclear phosphoprotein belonging to the WD-40 family. | Honoré, B., et al. 1994. Gene. 151: 291-6. PMID: 7828893
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PWP1 Antibody (B-2) | sc-166656 | 200 µg/ml | $322.00 | |||
PWP1 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-529020 | 200 µg Ab; 10 µg BP | $361.00 | |||
PWP1 Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-521695 | 200 µg Ab, 40 µg BP | $361.00 | |||
PWP1 Antibody (B-2): m-IgG2a BP-HRP Bundle | sc-547237 | 200 µg Ab; 10 µg BP | $361.00 | |||
PWP1 Antibody (B-2) AC | sc-166656 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PWP1 Antibody (B-2) HRP | sc-166656 HRP | 200 µg/ml | $322.00 | |||
PWP1 Antibody (B-2) FITC | sc-166656 FITC | 200 µg/ml | $336.00 | |||
PWP1 Antibody (B-2) PE | sc-166656 PE | 200 µg/ml | $349.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 488 | sc-166656 AF488 | 200 µg/ml | $364.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 546 | sc-166656 AF546 | 200 µg/ml | $364.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 594 | sc-166656 AF594 | 200 µg/ml | $364.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 647 | sc-166656 AF647 | 200 µg/ml | $364.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 680 | sc-166656 AF680 | 200 µg/ml | $364.00 | |||
PWP1 Antibody (B-2) Alexa Fluor® 790 | sc-166656 AF790 | 200 µg/ml | $364.00 |