PUMAα/β Antibody (G-3): sc-374223

PUMAα/β Antibody (G-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects PUMA alpha/beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PUMAα/β antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PUMA protein, also known as Bcl-2 binding component 3, plays a critical role in regulating apoptosis, particularly in response to cellular stress signals such as γ-radiation and glucocorticoid treatment. PUMA localizes in mitochondria, where PUMA interacts with other members of the Bcl-2 family to promote cytochrome c release, thereby initiating the apoptotic cascade. PUMA gene encodes several splice variants, including PUMAα, β, γ, and δ, with PUMAα and PUMAβ containing a crucial BH3 domain that facilitates binding to anti-apoptotic proteins like Bcl-2 and Bcl-xL. This interaction is vital for PUMA′s pro-apoptotic function, as PUMA helps neutralize the protective effects of these anti-apoptotic proteins, allowing apoptosis to proceed. Understanding PUMA′s function and localization is essential for elucidating apoptosis mechanisms and their implications in cancer therapy and other diseases where cell death regulation is disrupted.

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PUMAα/β Antibody (G-3) References:

1. PUMA induces the rapid apoptosis of colorectal cancer cells.  |  Yu, J., et al. 2001. Mol Cell. 7: 673-82. PMID: 11463391
2. PUMA, a novel proapoptotic gene, is induced by p53.  |  Nakano, K. and Vousden, KH. 2001. Mol Cell. 7: 683-94. PMID: 11463392
3. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.  |  Han, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 11318-23. PMID: 11572983
4. BH3-only proteins - evolutionarily conserved proapoptotic Bcl-2 family members essential for initiating programmed cell death.  |  Bouillet, P. and Strasser, A. 2002. J Cell Sci. 115: 1567-74. PMID: 11950875
5. Puma is an essential mediator of p53-dependent and -independent apoptotic pathways.  |  Jeffers, JR., et al. 2003. Cancer Cell. 4: 321-8. PMID: 14585359
6. Suppression of tumorigenesis by the p53 target PUMA.  |  Hemann, MT., et al. 2004. Proc Natl Acad Sci U S A. 101: 9333-8. PMID: 15192153
7. p53 activation domain 1 is essential for PUMA upregulation and p53-mediated neuronal cell death.  |  Cregan, SP., et al. 2004. J Neurosci. 24: 10003-12. PMID: 15525786
8. The first alpha helix of Bax plays a necessary role in its ligand-induced activation by the BH3-only proteins Bid and PUMA.  |  Cartron, PF., et al. 2004. Mol Cell. 16: 807-18. PMID: 15574335
9. BH3-only proteins Puma and Bim are rate-limiting for gamma-radiation- and glucocorticoid-induced apoptosis of lymphoid cells in vivo.  |  Erlacher, M., et al. 2005. Blood. 106: 4131-8. PMID: 16118324
10. Induction of PUMA-alpha and down-regulation of PUMA-beta expression is associated with benzo(a)pyrene-induced apoptosis in MCF-7 cells.  |  Tampio, M., et al. 2009. Toxicol Lett. 188: 214-22. PMID: 19397966
11. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells.  |  Kang, JW., et al. 2021. Signal Transduct Target Ther. 6: 129. PMID: 33785736
12. The carboxyl-terminal sequence of PUMA binds to both anti-apoptotic proteins and membranes.  |  Pemberton, JM., et al. 2023. Elife. 12: PMID: 37078707

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PUMAα/β Antibody (G-3) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-PUMAα/β Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PUMAα/β Antibody (G-3) Specifications

Alternate Product Name: PUMA

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 57-193

Epitope Target: PUMAα

Epitope Species: human

Product Reactivity/Detection: PUMAα and PUMAβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for PUMAα/β Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PUMAα antibody is PUMAα Antibody (B-6): sc-377015.