PUMAα Antibody (B-6): sc-377015

PUMAα Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PUMA alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PUMAα (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PUMA protein, also known as Bcl-2 binding component 3, plays a critical role in regulating apoptosis, particularly in response to cellular stressors such as γ-radiation and glucocorticoids. PUMA is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that initiates apoptosis through a Bax- and mitochondria-dependent pathway, making PUMA essential for maintaining cellular homeostasis and preventing tumorigenesis. PUMA gene encodes several splice variants, including PUMAα, β, γ, and δ, with PUMAα and PUMAβ containing the crucial BH3 domain that facilitates interaction with anti-apoptotic proteins like Bcl-2 and Bcl-xL. This interaction is vital for the pro-apoptotic function of PUMA, as PUMA helps transmit death signals to the mitochondria, thereby promoting cell death in lymphoid cells. Understanding PUMA function and regulation is important for developing therapeutic strategies targeting apoptosis in cancer and other diseases.

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PUMAα Antibody (B-6) References:

1. PUMA induces the rapid apoptosis of colorectal cancer cells.  |  Yu, J., et al. 2001. Mol Cell. 7: 673-82. PMID: 11463391
2. PUMA, a novel proapoptotic gene, is induced by p53.  |  Nakano, K. and Vousden, KH. 2001. Mol Cell. 7: 683-94. PMID: 11463392
3. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.  |  Han, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 11318-23. PMID: 11572983
4. BH3-only proteins - evolutionarily conserved proapoptotic Bcl-2 family members essential for initiating programmed cell death.  |  Bouillet, P. and Strasser, A. 2002. J Cell Sci. 115: 1567-74. PMID: 11950875
5. Puma is an essential mediator of p53-dependent and -independent apoptotic pathways.  |  Jeffers, JR., et al. 2003. Cancer Cell. 4: 321-8. PMID: 14585359
6. Suppression of tumorigenesis by the p53 target PUMA.  |  Hemann, MT., et al. 2004. Proc Natl Acad Sci U S A. 101: 9333-8. PMID: 15192153
7. p53 activation domain 1 is essential for PUMA upregulation and p53-mediated neuronal cell death.  |  Cregan, SP., et al. 2004. J Neurosci. 24: 10003-12. PMID: 15525786
8. The first alpha helix of Bax plays a necessary role in its ligand-induced activation by the BH3-only proteins Bid and PUMA.  |  Cartron, PF., et al. 2004. Mol Cell. 16: 807-18. PMID: 15574335
9. BH3-only proteins Puma and Bim are rate-limiting for gamma-radiation- and glucocorticoid-induced apoptosis of lymphoid cells in vivo.  |  Erlacher, M., et al. 2005. Blood. 106: 4131-8. PMID: 16118324
10. Regulation of PUMA-alpha by p53 in cisplatin-induced renal cell apoptosis.  |  Jiang, M., et al. 2006. Oncogene. 25: 4056-66. PMID: 16491117
11. Induction of PUMA-alpha and down-regulation of PUMA-beta expression is associated with benzo(a)pyrene-induced apoptosis in MCF-7 cells.  |  Tampio, M., et al. 2009. Toxicol Lett. 188: 214-22. PMID: 19397966
12. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells.  |  Kang, JW., et al. 2021. Signal Transduct Target Ther. 6: 129. PMID: 33785736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PUMAα Antibody (B-6) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-PUMAα Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PUMAα Antibody (B-6) Specifications

Alternate Product Name: PUMA alpha

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-29

Epitope Target: PUMAα

Epitope Species: human

Product Reactivity/Detection: PUMAα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for PUMAα Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PUMAα/β antibody is PUMAα/β Antibody (G-3): sc-374223.