PTRH2 Antibody (E-7): sc-518195
- PTRH2 Antibody (E-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 143-155 near the C-terminus of PTRH2 of human origin
- recommended for detection of PTRH2 of human origin by WB, IP, IF and ELISA
- Anti-PTRH2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for PTRH2 Antibody (E-7) for WB applications. This reagent is now offered in a bundle with PTRH2 Antibody (E-7) (see ordering information below).
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PTRH2 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PTRH2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PTRH2 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PTRH2, also known as peptidyl-tRNA hydrolase 2 or BIT1 (Bcl-2 inhibitor of transcription 1), is a crucial 179 amino acid mitochondrial protein that plays a significant role in the regulation of apoptosis. During the apoptotic process, PTRH2 is released from the mitochondria into the cytoplasm, where PTRH2 interacts with transcriptional regulators TLE5 and TLE1, promoting caspase-independent cell death. This function provides an alternative pathway for cell death that can bypass traditional caspase-dependent mechanisms, which may benefit certain pathological conditions where apoptosis is dysregulated. Additionally, PTRH2 acts on peptidyl-tRNAs released from ribosomes during protein synthesis, highlighting PTRH2′s role in both mitochondrial function and cellular stress responses. PTRH2′s structure includes an N-terminal mitochondrial localization signal and a C-terminal UPF0099 domain, which are essential for proper localization and function within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PTRH2 Antibody (E-7) References:
- Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. | Lai, CH., et al. 2000. Genome Res. 10: 703-13. PMID: 10810093
- Crystal structure of a human peptidyl-tRNA hydrolase reveals a new fold and suggests basis for a bifunctional activity. | De Pereda, JM., et al. 2004. J Biol Chem. 279: 8111-5. PMID: 14660562
- A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors. | Jan, Y., et al. 2004. Cell. 116: 751-62. PMID: 15006356
- Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA. | Gonzalez de Valdivia, EI. and Isaksson, LA. 2005. FEBS J. 272: 5306-16. PMID: 16218960
- Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis. | Selvaraj, M., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 913-5. PMID: 16946478
- Yeast Pth2 is a UBL domain-binding protein that participates in the ubiquitin-proteasome pathway. | Ishii, T., et al. 2006. EMBO J. 25: 5492-503. PMID: 17082762
- Induction of Anoikis following myoblast transplantation into SCID mouse muscles requires the Bit1 and FADD pathways. | Bouchentouf, M., et al. 2007. Am J Transplant. 7: 1491-505. PMID: 17511679
- Protein kinase D is a positive regulator of Bit1 apoptotic function. | Biliran, H., et al. 2008. J Biol Chem. 283: 28029-37. PMID: 18703509
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTRH2 Antibody (E-7) | sc-518195 | 200 µg/ml | $316.00 | |||
PTRH2 Antibody (E-7): m-IgG1 BP-HRP Bundle | sc-550987 | 200 µg Ab; 20 µg BP | $354.00 | |||
PTRH2 Antibody (E-7) AC | sc-518195 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PTRH2 Antibody (E-7) HRP | sc-518195 HRP | 200 µg/ml | $316.00 | |||
PTRH2 Antibody (E-7) FITC | sc-518195 FITC | 200 µg/ml | $330.00 | |||
PTRH2 Antibody (E-7) PE | sc-518195 PE | 200 µg/ml | $343.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 488 | sc-518195 AF488 | 200 µg/ml | $357.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 546 | sc-518195 AF546 | 200 µg/ml | $357.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 594 | sc-518195 AF594 | 200 µg/ml | $357.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 647 | sc-518195 AF647 | 200 µg/ml | $357.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 680 | sc-518195 AF680 | 200 µg/ml | $357.00 | |||
PTRH2 Antibody (E-7) Alexa Fluor® 790 | sc-518195 AF790 | 200 µg/ml | $357.00 |