PTPS Antibody (D-8): sc-514628
- PTPS Antibody (D-8) is a mouse monoclonal IgG1 κ PTPS antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-145 representing full length PTPS of human origin
- PTPS Antibody (D-8) is recommended for detection of PTPS of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PTPS Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PTPS Antibody (D-8) for WB applications. These reagents are now offered in bundles with PTPS Antibody (D-8) (see ordering information below).
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SEE ALSO...
PTPS Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PTPS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PTPS (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PTPS protein plays a crucial role in the biosynthesis of tetrahydrobiopterin (BH4), a vital cofactor necessary for the activity of several hydroxylases involved in the degradation of phenylalanine and the synthesis of neurotransmitters such as catecholamines and serotonin. BH4 is also essential for nitric oxide synthase, and deficiency can lead to serious metabolic disorders, including hyperphenylalaninemia and depletion of monoamine neurotransmitters, often resulting from mutations in the PTPS gene. PTPS structure is particularly important, as the active site features a pterin-anchoring glutamic acid residue (Glu A107) alongside a zinc-binding site and a catalytic triad composed of cysteine, aspartate, and histidine residues. This configuration facilitates a series of critical reactions, including triphosphate elimination and stereospecific reductions, which are essential for enzyme function. Additionally, post-translational modifications, such as phosphorylation of Ser 19 by cGMP-dependent protein kinase type II, are vital for maintaining normal PTPS activity, highlighting the intricate regulation of this enzyme in physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PTPS Antibody (D-8) References:
- Crystallographic and kinetic investigations on the mechanism of 6-pyruvoyl tetrahydropterin synthase. | Ploom, T., et al. 1999. J Mol Biol. 286: 851-60. PMID: 10024455
- Serine 19 of human 6-pyruvoyltetrahydropterin synthase is phosphorylated by cGMP protein kinase II. | Scherer-Oppliger, T., et al. 1999. J Biol Chem. 274: 31341-8. PMID: 10531334
- Human 6-pyruvoyltetrahydropterin synthase: cDNA cloning and heterologous expression of the recombinant enzyme. | Thöny, B., et al. 1992. Biochem Biophys Res Commun. 189: 1437-43. PMID: 1282802
- Chromosomal location of two human genes encoding tetrahydrobiopterin-metabolizing enzymes: 6-pyruvoyl-tetrahydropterin synthase maps to 11q22.3-q23.3, and pterin-4 alpha-carbinolamine dehydratase maps to 10q22. | Thöny, B., et al. 1994. Genomics. 19: 365-8. PMID: 8188266
- Chromosomal localization, genomic structure and characterization of the human gene and a retropseudogene for 6-pyruvoyltetrahydropterin synthase. | Kluge, C., et al. 1996. Eur J Biochem. 240: 477-84. PMID: 8841415
- Retrovirus-mediated double transduction of the GTPCH and PTPS genes allows 6-pyruvoyltetrahydropterin synthase-deficient human fibroblasts to synthesize and release tetrahydrobiopterin. | Laufs, S., et al. 1998. J Neurochem. 71: 33-40. PMID: 9648848
- Regulation of 6-pyruvoyltetrahydropterin synthase activity and messenger RNA abundance in human vascular endothelial cells. | Linscheid, P., et al. 1998. Circulation. 98: 1703-6. PMID: 9788822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTPS Antibody (D-8) | sc-514628 | 200 µg/ml | $322.00 | |||
PTPS Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-531181 | 200 µg Ab; 10 µg BP | $361.00 | |||
PTPS Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-524796 | 200 µg Ab, 40 µg BP | $361.00 | |||
PTPS Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-544426 | 200 µg Ab; 20 µg BP | $361.00 | |||
PTPS Antibody (D-8) AC | sc-514628 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PTPS Antibody (D-8) HRP | sc-514628 HRP | 200 µg/ml | $322.00 | |||
PTPS Antibody (D-8) FITC | sc-514628 FITC | 200 µg/ml | $336.00 | |||
PTPS Antibody (D-8) PE | sc-514628 PE | 200 µg/ml | $349.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 488 | sc-514628 AF488 | 200 µg/ml | $364.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 546 | sc-514628 AF546 | 200 µg/ml | $364.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 594 | sc-514628 AF594 | 200 µg/ml | $364.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 647 | sc-514628 AF647 | 200 µg/ml | $364.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 680 | sc-514628 AF680 | 200 µg/ml | $364.00 | |||
PTPS Antibody (D-8) Alexa Fluor® 790 | sc-514628 AF790 | 200 µg/ml | $364.00 |