PTP IA-2 Antibody (A-5): sc-390101
- PTP IA-2 Antibody (A-5) is a mouse monoclonal IgG2a κ PTP IA-2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 723-763 mapping within an internal region of PTP IA-2 of human origin
- PTP IA-2 Antibody (A-5) is recommended for detection of PTP IA-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
- Anti-PTP IA-2 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PTP IA-2 Antibody (A-5) for WB applications. These reagents are now offered in bundles with PTP IA-2 Antibody (A-5) (see ordering information below).
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PTP IA-2 Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects PTP IA-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PTP IA-2 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PTP IA-2, also known as PTPRN, IA2, ICA512, or RPTPN, is a receptor-type protein tyrosine phosphatase that plays a crucial role in regulating secretory granule exocytosis, particularly in neuroendocrine cells such as pancreatic islet cells. PTP IA-2 is characterized by a transmembrane structure, which includes an extracellular N-terminus, an intracellular phosphatase domain, and a transmembrane region, allowing effective mediation of signaling pathways involved in insulin secretion. PTP IA-2 localization to secretory granules is vital for function, as this facilitates precise hormone release in response to physiological stimuli. PTP IA-2 is recognized as an autoantigen in insulin-dependent diabetes mellitus (IDDM), and autoantibodies against PTP IA-2 serve as significant biomarkers for diabetes diagnosis, highlighting importance in both clinical and research settings.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PTP IA-2 Antibody (A-5) References:
- Expression and physicochemical characterization of an extracellular segment of the receptor protein tyrosine phosphatase IA-2. | Primo, ME., et al. 2006. Biochim Biophys Acta. 1764: 174-81. PMID: 16413232
- Monoclonal antibody 76F distinguishes IA-2 from IA-2beta and overlaps an autoantibody epitope. | Piquer, S., et al. 2006. J Autoimmun. 26: 215-22. PMID: 16503116
- Genome-wide review of transcriptional complexity in mouse protein kinases and phosphatases. | Forrest, AR., et al. 2006. Genome Biol. 7: R5. PMID: 16507138
- Alternative splicing of G6PC2, the gene coding for the islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP), results in differential expression in human thymus and spleen compared with pancreas. | Dogra, RS., et al. 2006. Diabetologia. 49: 953-7. PMID: 16520917
- Synergy of glucose and growth hormone signalling in islet cells through ICA512 and STAT5. | Mziaut, H., et al. 2006. Nat Cell Biol. 8: 435-45. PMID: 16622421
- Engineering and expression of the intracellular domain of insulinoma-associated tyrosine phosphatase (IA-2ic), a type 1 diabetes autoantigen, in plants. | Mett, V., et al. 2007. Transgenic Res. 16: 77-84. PMID: 17103024
- MHC class I chain-related gene-A is associated with IA2 and IAA but not GAD in Swedish type 1 diabetes mellitus. | Gupta, M., et al. 2006. Ann N Y Acad Sci. 1079: 229-39. PMID: 17130560
- Crystal structure of the major diabetes autoantigen insulinoma-associated protein 2 reveals distinctive immune epitopes. | Kim, SJ., et al. 2007. Diabetes. 56: 41-8. PMID: 17192463
- Immunization of HLA class I transgenic mice identifies autoantigenic epitopes eliciting dominant responses in type 1 diabetes patients. | Blancou, P., et al. 2007. J Immunol. 178: 7458-66. PMID: 17513797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTP IA-2 Antibody (A-5) | sc-390101 | 200 µg/ml | $316.00 | |||
PTP IA-2 Antibody (A-5): m-IgG2a BP-HRP Bundle | sc-545956 | 200 µg Ab; 10 µg BP | $354.00 | |||
PTP IA-2 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-551264 | 200 µg Ab; 40 µg BP | $354.00 | |||
PTP IA-2 Antibody (A-5) AC | sc-390101 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PTP IA-2 Antibody (A-5) HRP | sc-390101 HRP | 200 µg/ml | $316.00 | |||
PTP IA-2 Antibody (A-5) FITC | sc-390101 FITC | 200 µg/ml | $330.00 | |||
PTP IA-2 Antibody (A-5) PE | sc-390101 PE | 200 µg/ml | $343.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 488 | sc-390101 AF488 | 200 µg/ml | $357.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 546 | sc-390101 AF546 | 200 µg/ml | $357.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 594 | sc-390101 AF594 | 200 µg/ml | $357.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 647 | sc-390101 AF647 | 200 µg/ml | $357.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 680 | sc-390101 AF680 | 200 µg/ml | $357.00 | |||
PTP IA-2 Antibody (A-5) Alexa Fluor® 790 | sc-390101 AF790 | 200 µg/ml | $357.00 |