PSMD5 Antibody (D-11): sc-390751
- PSMD5 Antibody (D-11) is a mouse monoclonal IgG2a κ PSMD5 antibody provided at 200 µg/ml
- raised against amino acids 205-504 mapping at the C-terminus of PSMD5 of human origin
- PSMD5 Antibody (D-11) is recommended for detection of PSMD5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PSMD5 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PSMD5 Antibody (D-11) for WB applications. These reagents are now offered in bundles with PSMD5 Antibody (D-11) (see ordering information below).
PSMD5 Antibody (D-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects PSMD5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PSMD5 (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSMD5 protein plays a crucial role in the 26S proteasome, a vital protease complex responsible for the selective degradation of ubiquitinated proteins, which is essential for maintaining cellular homeostasis and regulating various cellular processes, including the cell cycle, apoptosis, and responses to oxidative stress. Specifically, PSMD5 is a subunit of the 19S regulatory particle of the 26S proteasome, where PSMD5 forms a heterotrimer with PSMC1 and PSMC2, facilitating the recognition and unfolding of ubiquitinated substrates. The presence of nine di-leucine repeats and a motif resembling a tyrosine-based motif in PSMD5 suggests involvement in the trafficking and targeting of proteins for degradation, highlighting importance in cellular protein quality control and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PSMD5 Antibody (D-11) References:
- Mapping subunit contacts in the regulatory complex of the 26 S proteasome. S2 and S5b form a tetramer with ATPase subunits S4 and S7. | Gorbea, C., et al. 2000. J Biol Chem. 275: 875-82. PMID: 10625621
- The proteasome regulatory particle subunit Rpn6 is required for Drosophila development and interacts physically with signalosome subunit Alien/CSN2. | Lier, S. and Paululat, A. 2002. Gene. 298: 109-19. PMID: 12426099
- Large- and small-scale purification of mammalian 26S proteasomes. | Hirano, Y., et al. 2005. Methods Enzymol. 399: 227-40. PMID: 16338359
- The testis-specific proteasome subunit Prosalpha6T of D. melanogaster is required for individualization and nuclear maturation during spermatogenesis. | Zhong, L. and Belote, JM. 2007. Development. 134: 3517-25. PMID: 17728345
- Molecular cloning and expression of a 26 S protease subunit enriched in dileucine repeats. | Deveraux, Q., et al. 1995. J Biol Chem. 270: 23726-9. PMID: 7559544
- Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. | Nomura, N., et al. 1994. DNA Res. 1: 223-9. PMID: 7584044
- A 26 S protease subunit that binds ubiquitin conjugates. | Deveraux, Q., et al. 1994. J Biol Chem. 269: 7059-61. PMID: 8125911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSMD5 Antibody (D-11) | sc-390751 | 200 µg/ml | $316.00 | |||
PSMD5 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-530247 | 200 µg Ab; 10 µg BP | $354.00 | |||
PSMD5 Antibody (D-11): m-IgGκ BP-HRP Bundle | sc-523664 | 200 µg Ab, 40 µg BP | $354.00 | |||
PSMD5 Antibody (D-11) AC | sc-390751 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PSMD5 Antibody (D-11) HRP | sc-390751 HRP | 200 µg/ml | $316.00 | |||
PSMD5 Antibody (D-11) FITC | sc-390751 FITC | 200 µg/ml | $330.00 | |||
PSMD5 Antibody (D-11) PE | sc-390751 PE | 200 µg/ml | $343.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 488 | sc-390751 AF488 | 200 µg/ml | $357.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 546 | sc-390751 AF546 | 200 µg/ml | $357.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 594 | sc-390751 AF594 | 200 µg/ml | $357.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 647 | sc-390751 AF647 | 200 µg/ml | $357.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 680 | sc-390751 AF680 | 200 µg/ml | $357.00 | |||
PSMD5 Antibody (D-11) Alexa Fluor® 790 | sc-390751 AF790 | 200 µg/ml | $357.00 |