PSMD12 Antibody (H-3): sc-398279
- PSMD12 Antibody (H-3) is a mouse monoclonal IgG1 κ PSMD12 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of PSMD12 of human origin
- PSMD12 Antibody (H-3) is recommended for detection of PSMD12 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-PSMD12 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PSMD12 Antibody (H-3) for WB applications. These reagents are now offered in bundles with PSMD12 Antibody (H-3) (see ordering information below).
PSMD12 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSMD12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PSMD12 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSMD12, also known as p55 or Rpn5, plays a crucial role as a regulatory subunit of the 26S proteasome, which is essential for the selective degradation of ubiquitinated proteins within eukaryotic cells. This process is vital for maintaining cellular homeostasis, regulating protein levels, and facilitating the removal of damaged or misfolded proteins, thereby preventing cellular stress and potential disease states. The 26S proteasome is composed of a 20S core particle that houses the proteolytic activity and two 19S regulatory particles that recognize and unfold ubiquitinated substrates. PSMD12, consisting of 456 amino acids and belonging to the proteasome subunit p55 family, contains a PCI domain that is integral to its function within the PA700 complex, further underscoring its importance in proteasomal activity and cellular regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PSMD12 Antibody (H-3) References:
- Using yeast to place human genes in functional categories. | Zhang, N., et al. 2003. Gene. 303: 121-9. PMID: 12559573
- Mass spectrometric analysis of expression of ATPase subunits encoded by duplicated genes in the 19S regulatory particle of rice 26S proteasome. | Shibahara, T., et al. 2004. Arch Biochem Biophys. 421: 34-41. PMID: 14678782
- Post-translationally modified S12, absent in transformed breast epithelial cells, is not associated with the 26S proteasome and is induced by proteasome inhibitor. | Thompson, HG., et al. 2004. Int J Cancer. 111: 338-47. PMID: 15221960
- The up-regulation of proteasome subunits and lysosomal proteases in hepatocellular carcinomas of the HBx gene knockin transgenic mice. | Cui, F., et al. 2006. Proteomics. 6: 498-504. PMID: 16317774
- Effects of tumor necrosis factor-alpha on the 26S proteasome and 19S regulator in skeletal muscle of severely scalded mice. | Tan, Y., et al. 2006. J Burn Care Res. 27: 226-33. PMID: 16566573
- Prostaglandin J2 alters pro-survival and pro-death gene expression patterns and 26 S proteasome assembly in human neuroblastoma cells. | Wang, Z., et al. 2006. J Biol Chem. 281: 21377-21386. PMID: 16737963
- Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics. | Deng, S., et al. 2007. Breast Cancer Res Treat. 104: 21-30. PMID: 17004105
- Thyroid hormone-related regulation of gene expression in human fatty liver. | Pihlajamäki, J., et al. 2009. J Clin Endocrinol Metab. 94: 3521-9. PMID: 19549744
- CDNA cloning of p112, the largest regulatory subunit of the human 26s proteasome, and functional analysis of its yeast homologue, sen3p. | Yokota, K., et al. 1996. Mol Biol Cell. 7: 853-70. PMID: 8816993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSMD12 Antibody (H-3) | sc-398279 | 200 µg/ml | $322.00 | |||
PSMD12 Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-527760 | 200 µg Ab; 10 µg BP | $361.00 | |||
PSMD12 Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-533133 | 200 µg Ab; 20 µg BP | $361.00 | |||
PSMD12 Antibody (H-3) AC | sc-398279 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PSMD12 Antibody (H-3) HRP | sc-398279 HRP | 200 µg/ml | $322.00 | |||
PSMD12 Antibody (H-3) FITC | sc-398279 FITC | 200 µg/ml | $336.00 | |||
PSMD12 Antibody (H-3) PE | sc-398279 PE | 200 µg/ml | $349.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 488 | sc-398279 AF488 | 200 µg/ml | $364.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 546 | sc-398279 AF546 | 200 µg/ml | $364.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 594 | sc-398279 AF594 | 200 µg/ml | $364.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 647 | sc-398279 AF647 | 200 µg/ml | $364.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 680 | sc-398279 AF680 | 200 µg/ml | $364.00 | |||
PSMD12 Antibody (H-3) Alexa Fluor® 790 | sc-398279 AF790 | 200 µg/ml | $364.00 |