pS2 Antibody (A-10): sc-271464

pS2 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects pS2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-pS2 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. pS2 protein, also known as trefoil factor 1, plays a crucial role in mucosal healing and is secreted throughout the gastrointestinal tract. Three intrachain disulfide bonds create a characteristic trefoil motif in pS2′s structure, which is essential for stability and function. This structural integrity allows pS2 to resist proteolytic degradation, ensuring availability at sites of injury in the gut, where pS2 promotes epithelial cell migration and repair. pS2 is highly expressed in normal gastric mucosa but absent in normal breast tissue, although estrogen can induce pS2 in MCF7 cells. pS2 has been implicated in various carcinomas, including those of the breast, pancreas, and stomach, highlighting potential as a biomarker for cancer progression. The gene encoding pS2 is located on human chromosome 21q22.3, emphasizing significance in human health and disease.

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pS2 Antibody (A-10) References:

1. Two antiestrogens affect differently chromatin remodeling of trefoil factor 1 (pS2) gene and the fate of estrogen receptor in MCF7 cells.  |  Giamarchi, C., et al. 2002. Biochim Biophys Acta. 1578: 12-20. PMID: 12393183
2. Selective abrogation of the proinvasive activity of the trefoil peptides pS2 and spasmolytic polypeptide by disruption of the EGF receptor signaling pathways in kidney and colonic cancer cells.  |  Rodrigues, S., et al. 2003. Oncogene. 22: 4488-97. PMID: 12881705
3. pS2 protein and steroid hormone receptors in invasive breast carcinomas.  |  Koerner, FC., et al. 1992. Int J Cancer. 52: 183-8. PMID: 1521907
4. SET-mediated promoter hypoacetylation is a prerequisite for coactivation of the estrogen-responsive pS2 gene by PRMT1.  |  Wagner, S., et al. 2006. J Biol Chem. 281: 27242-50. PMID: 16861234
5. Clinical significance of the estrogen regulated pS2 protein in mammary tumors.  |  Pichon, MF. and Milgrom, E. 1993. Crit Rev Oncol Hematol. 15: 13-21. PMID: 8240704
6. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression.  |  Gött, P., et al. 1996. Eur J Hum Genet. 4: 308-15. PMID: 9043862
7. Trefoil proteins: their role in normal and malignant cells.  |  May, FE. and Westley, BR. 1997. J Pathol. 183: 4-7. PMID: 9370940
8. Intestinal trefoil factor (TFF 3) and pS2 (TFF 1), but not spasmolytic polypeptide (TFF 2) mRNAs are co-expressed in normal, hyperplastic, and neoplastic human breast epithelium.  |  Poulsom, R., et al. 1997. J Pathol. 183: 30-8. PMID: 9370944
9. Trefoil peptides: from structure to function.  |  Thim, L. 1997. Cell Mol Life Sci. 53: 888-903. PMID: 9447240
10. Dimerization of human pS2 (TFF1) plays a key role in its protective/healing effects.  |  Marchbank, T., et al. 1998. J Pathol. 185: 153-8. PMID: 9713341
11. The pS2/TFF1 trefoil factor, from basic research to clinical applications.  |  Ribieras, S., et al. 1998. Biochim Biophys Acta. 1378: F61-77. PMID: 9739760
12. Growth factors and the gastrointestinal tract.  |  Murphy, MS. 1998. Nutrition. 14: 771-4. PMID: 9785359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pS2 Antibody (A-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-pS2 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pS2 Antibody (A-10) Specifications

Alternate Product Name: pS2

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-84

Epitope Target: pS2

Epitope Species: human

Product Reactivity/Detection: precursor and mature pS2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for pS2 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin