PRR5 Antibody (A-7): sc-390469
- PRR5 Antibody (A-7) is a mouse monoclonal IgG2b κ PRR5 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 253-270 within an internal region of PRR5 of human origin
- PRR5 Antibody (A-7) is recommended for detection of PRR5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PRR5 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PRR5 Antibody (A-7) for WB applications. These reagents are now offered in bundles with PRR5 Antibody (A-7) (see ordering information below).
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PRR5 Antibody (A-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects PRR5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PRR5 antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRR5, also known as Protein observed with Rictor-1 (Protor-1), is a 388 amino acid protein that serves as a crucial subunit of the mammalian target of rapamycin complex 2 (mTORC2), a multimeric kinase complex that plays a vital role in regulating the actin cytoskeleton and phosphorylating key proteins such as Akt1, serum/glucocorticoid-regulated kinase (SGK), and protein kinase C alpha (PKC α). PRR5′s importance lies in its involvement in the modulation of platelet-derived growth factor signaling and the regulation of PDGFR-β expression, which are critical for various cellular processes, including cell growth and survival. PRR5 is implicated in cancer biology, as the gene encoding PRR5 is located in a genomic region that frequently experiences loss of heterozygosity in breast cancer, suggesting a potential role as a tumor suppressor. PRR5 exists in four isoforms due to alternative splicing, which may contribute to functional diversity and regulatory mechanisms within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRR5 Antibody (A-7) References:
- PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas. | Johnstone, CN., et al. 2005. Genomics. 85: 338-51. PMID: 15718101
- Identification of Protor as a novel Rictor-binding component of mTOR complex-2. | Pearce, LR., et al. 2007. Biochem J. 405: 513-22. PMID: 17461779
- Arabidopsis clock-associated pseudo-response regulators PRR9, PRR7 and PRR5 coordinately and positively regulate flowering time through the canonical CONSTANS-dependent photoperiodic pathway. | Nakamichi, N., et al. 2007. Plant Cell Physiol. 48: 822-32. PMID: 17504813
- PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor beta expression and signaling. | Woo, SY., et al. 2007. J Biol Chem. 282: 25604-12. PMID: 17599906
- PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. | Thedieck, K., et al. 2007. PLoS One. 2: e1217. PMID: 18030348
- Insight into missing genetic links between two evening-expressed pseudo-response regulator genes TOC1 and PRR5 in the circadian clock-controlled circuitry in Arabidopsis thaliana. | Ito, S., et al. 2008. Plant Cell Physiol. 49: 201-13. PMID: 18178585
- Characterization of genetic links between two clock-associated genes, GI and PRR5 in the current clock model of Arabidopsis thaliana. | Kawamura, H., et al. 2008. Biosci Biotechnol Biochem. 72: 2770-4. PMID: 18838788
- mTOR-what does it do? | Hall, MN. 2008. Transplant Proc. 40: S5-8. PMID: 19100909
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRR5 Antibody (A-7) | sc-390469 | 200 µg/ml | $322.00 | |||
PRR5 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-523544 | 200 µg Ab, 40 µg BP | $361.00 | |||
PRR5 Antibody (A-7): m-IgG2b BP-HRP Bundle | sc-549124 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRR5 Antibody (A-7) AC | sc-390469 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PRR5 Antibody (A-7) HRP | sc-390469 HRP | 200 µg/ml | $322.00 | |||
PRR5 Antibody (A-7) FITC | sc-390469 FITC | 200 µg/ml | $336.00 | |||
PRR5 Antibody (A-7) PE | sc-390469 PE | 200 µg/ml | $349.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 488 | sc-390469 AF488 | 200 µg/ml | $364.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 546 | sc-390469 AF546 | 200 µg/ml | $364.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 594 | sc-390469 AF594 | 200 µg/ml | $364.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 647 | sc-390469 AF647 | 200 µg/ml | $364.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 680 | sc-390469 AF680 | 200 µg/ml | $364.00 | |||
PRR5 Antibody (A-7) Alexa Fluor® 790 | sc-390469 AF790 | 200 µg/ml | $364.00 | |||
PRR5 (A-7) Neutralizing Peptide | sc-390469 P | 100 µg/0.5 ml | $69.00 |