PRP8 Antibody (F-6): sc-55534

PRP8 Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects PRP8 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PRP8, also known as pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein that serves as a central component of the spliceosome′s catalytic core, playing a crucial role in pre-mRNA splicing. PRP8 is essential for transesterification reactions that facilitate spliceosome-induced pre-mRNA splicing, particularly through interaction with the GU dinucleotide at the 5′ splice site, forming a specific UV-inducible cross-link. PRP8′s functional interaction with the 3′ splice site significantly influences the efficiency of the second catalytic step in splicing. PRP8 is involved in the first transesterification step, as mutations that disrupt the negative regulation of PRP28 or PRP44/Brr2 can block U4 activation, highlighting PRP8′s critical role in spliceosome assembly and function. PRP8 interacts with a conserved region of U6, which is vital for the formation of the spliceosome′s catalytic core, underscoring PRP8′s importance in gene expression regulation.

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PRP8 Antibody (F-6) References:

1. Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome.  |  Kuhn, AN., et al. 1999. Mol Cell. 3: 65-75. PMID: 10024880
2. Roles of PRP8 protein in the assembly of splicing complexes.  |  Brown, JD. and Beggs, JD. 1992. EMBO J. 11: 3721-9. PMID: 1396567
3. Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.  |  Schneider, S., et al. 2004. J Biol Chem. 279: 8617-26. PMID: 14688266
4. Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants.  |  Zhang, L., et al. 2007. Protein Sci. 16: 1024-31. PMID: 17473007
5. Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.  |  Kershaw, CJ., et al. 2009. RNA. 15: 1292-304. PMID: 19447917
6. A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.  |  Schellenberg, MJ., et al. 2013. Nat Struct Mol Biol. 20: 728-34. PMID: 23686287
7. Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.  |  Mayerle, M. and Guthrie, C. 2016. RNA. 22: 793-809. PMID: 26968627
8. Cisplatin protects mice from challenge of Cryptococcus neoformans by targeting the Prp8 intein.  |  Li, Z., et al. 2019. Emerg Microbes Infect. 8: 895-908. PMID: 31223062
9. Small-molecule inhibitors for the Prp8 intein as antifungal agents.  |  Li, Z., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33397721
10. Ecd promotes U5 snRNP maturation and Prp8 stability.  |  Erkelenz, S., et al. 2021. Nucleic Acids Res. 49: 1688-1707. PMID: 33444449

The preferred PRP8 antibody is PRP8 Antibody (E-5): sc-55533.