Profilin-1 Antibody (B-10): sc-137235

Profilin-1 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Profilin-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Profilin-1 antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Profilin-1 plays a crucial role in regulating actin polymerization by binding to and sequestering actin monomers, which is essential for maintaining cellular structure and facilitating various cellular processes such as motility and division. Profilin-1 acts as a nucleotide exchange factor, charging actin with ATP after forming a 1:1 complex with the actin monomer, thereby promoting dynamic remodeling of the cytoskeleton. Profilin-1 is predominantly expressed in tissues such as the lung, liver, placenta, and kidney, while Profilin-2 is highly expressed in the brain and skeletal muscle. Notably, Profilin-1 and Profilin-2 are encoded by separate genes located on chromosomes 17p13.2 and 3q25.1, respectively. In neuronal function, Profilin-1 co-localizes with proteins like dynamin I and synapsin in axonal and dendritic processes, suggesting involvement in synaptic function and plasticity. Additionally, Profilin-1 overexpression in endothelial cells has been linked to enhanced adhesion to fibronectin, indicating a potential role in cell adhesion mechanisms. Furthermore, in food allergies, plant-derived Profilin is recognized as a pan-allergen, with case studies revealing that individuals allergic to foods such as celery, carrots, zucchini, and peanuts may actually be reacting to Profilin proteins present in these items.

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Profilin-1 Antibody (B-10) References:

1. The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells.  |  Goldschmidt-Clermont, PJ., et al. 1992. Mol Biol Cell. 3: 1015-24. PMID: 1330091
2. Profilins constitute a novel family of functional plant pan-allergens.  |  Valenta, R., et al. 1992. J Exp Med. 175: 377-85. PMID: 1370681
3. Identification of the functional profilin gene, its localization to chromosome subband 17p13.3, and demonstration of its deletion in some patients with Miller-Dieker syndrome.  |  Kwiatkowski, DJ., et al. 1990. Am J Hum Genet. 46: 559-67. PMID: 1968707
4. RhoA/mDia-1/profilin-1 signaling targets microvascular endothelial dysfunction in diabetic retinopathy.  |  Lu, Q., et al. 2015. Graefes Arch Clin Exp Ophthalmol. 253: 669-80. PMID: 25791356
5. The role of profilin-1 in cardiovascular diseases.  |  Allen, A., et al. 2021. J Cell Sci. 134: PMID: 33961053
6. Effect of profilin-1 on the asymmetric dimethylarginine-induced vascular lesion-associated hypertension.  |  Ni, GH., et al. 2022. Kaohsiung J Med Sci. 38: 149-156. PMID: 34741409
7. Profilin 1 deficiency drives mitotic defects and reduces genome stability.  |  Scotto di Carlo, F., et al. 2023. Commun Biol. 6: 9. PMID: 36599901
8. Role of profilin-1 in vasculopathy induced by advanced glycation end products (AGEs).  |  Xie, Q., et al. 2023. J Diabetes Complications. 37: 108415. PMID: 36989867
9. Cloning and expression of a novel human profilin variant, profilin II.  |  Honoré, B., et al. 1993. FEBS Lett. 330: 151-5. PMID: 8365484
10. Report of the sixth international workshop on human chromosome 3 mapping 1995.  |  Naylor, SL., et al. 1996. Cytogenet Cell Genet. 72: 255-70. PMID: 8641130
11. Regulation of endothelial cell adhesion by profilin.  |  Moldovan, NI., et al. 1997. Curr Biol. 7: 24-30. PMID: 9024619
12. In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly.  |  Witke, W., et al. 1998. EMBO J. 17: 967-76. PMID: 9463375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Profilin-1 Antibody (B-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Profilin-1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Profilin-1 Antibody (B-10) Specifications

Alternate Product Name: Profilin-1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-37

Epitope Target: Profilin-1

Epitope Species: human

Product Reactivity/Detection: Profilin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Profilin-1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin