probasin Antibody (F-6): sc-393830

Probasin Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects probasin protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-probasin antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Probasin, a prostate-specific and androgen-regulated protein, plays a crucial role in the functional differentiation of prostatic epithelium by facilitating the production of tissue-specific secretory proteins. This production is significantly influenced by serum androgens, with probasin being a key player in this process. Notably, probasin exists in both secreted and nuclear forms and is predominantly expressed in the epithelial cells of the prostate. The regulation of probasin gene transcription by androgens is a complex process that requires the presence of two androgen receptor-binding sites located within the 5′-flanking region of the probasin gene. The cooperative binding of the androgen receptor to these sites is essential for the effective transcriptional activation of the probasin gene, highlighting probasin′s importance in prostate differentiation and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

probasin Antibody (F-6) References:

1. Isolation and characterization of mouse probasin: An androgen-regulated protein specifically expressed in the differentiated prostate.  |  Johnson, MA., et al. 2000. Prostate. 43: 255-62. PMID: 10861744
2. Rat probasin: structure and function of an outlier lipocalin.  |  Kasper, S. and Matusik, RJ. 2000. Biochim Biophys Acta. 1482: 249-58. PMID: 11058766
3. Use of the probasin promoter ARR2PB to express Bax in androgen receptor-positive prostate cancer cells.  |  Andriani, F., et al. 2001. J Natl Cancer Inst. 93: 1314-24. PMID: 11535706
4. Prostatic intraepithelial neoplasia and intestinal metaplasia in prostates of probasin-RAS transgenic mice.  |  Scherl, A., et al. 2004. Prostate. 59: 448-59. PMID: 15065094
5. Probasin promoter-driven expression of ID1 is not sufficient for carcinogenesis in rodent prostate.  |  Salomon, R., et al. 2009. J Histochem Cytochem. 57: 599-604. PMID: 19255251
6. A Probasin-MerCreMer BAC allows inducible recombination in the mouse prostate.  |  Birbach, A., et al. 2009. Genesis. 47: 757-64. PMID: 19830822
7. Use of PB-Cre4 mice for mosaic gene deletion.  |  Birbach, A. 2013. PLoS One. 8: e53501. PMID: 23308238
8. Cooperative binding of androgen receptors to two DNA sequences is required for androgen induction of the probasin gene.  |  Kasper, S., et al. 1994. J Biol Chem. 269: 31763-9. PMID: 7989349
9. Characterization of two cis-acting DNA elements involved in the androgen regulation of the probasin gene.  |  Rennie, PS., et al. 1993. Mol Endocrinol. 7: 23-36. PMID: 8446105
10. Initiation of secretory activity of rat prostatic epithelium in organ culture.  |  Lopes, ES., et al. 1996. Endocrinology. 137: 4225-34. PMID: 8828481
11. Large fragment of the probasin promoter targets high levels of transgene expression to the prostate of transgenic mice.  |  Yan, Y., et al. 1997. Prostate. 32: 129-39. PMID: 9215401
12. Development, progression, and androgen-dependence of prostate tumors in probasin-large T antigen transgenic mice: a model for prostate cancer.  |  Kasper, S., et al. 1998. Lab Invest. 78: 319-33. PMID: 9520945

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-probasin Antibody (F-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-probasin Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

probasin Antibody (F-6) Specifications

Alternate Product Name: probasin

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 133-158

Epitope Target: probasin

Epitope Species: mouse

Product Reactivity/Detection: probasin

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for probasin Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin