Pro-COL3A1 Antibody (A-1): sc-166333

Pro-COL3A1 Antibody (A-1) is a mouse monoclonal IgG3 antibody that detects Procollagen α1 Type III in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Procollagen α1 Type III antibody (A-1) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Procollagen α1 Type III is a crucial component of the extracellular matrix, primarily found in connective tissues, where Procollagen α1 Type III plays a significant role in providing structural support and tensile strength. Procollagen α1 Type III is essential for the formation of collagen fibers, which are vital for maintaining the integrity and elasticity of tissues such as skin, blood vessels, and organs. Proper assembly and function of Procollagen α1 Type III are critical, as defects in synthesis or structure can lead to various connective tissue disorders, highlighting Procollagen α1 Type III′s importance in both health and disease.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Pro-COL3A1 Antibody (A-1) References:

1. Large kindred with Ehlers-Danlos syndrome type IV due to a point mutation (G571S) in the COL3A1 gene of type III procollagen: low risk of pregnancy complications and unexpected longevity in some affected relatives.  |  Gilchrist, D., et al. 1999. Am J Med Genet. 82: 305-11. PMID: 10051163
2. Haploinsufficiency for one COL3A1 allele of type III procollagen results in a phenotype similar to the vascular form of Ehlers-Danlos syndrome, Ehlers-Danlos syndrome type IV.  |  Schwarze, U., et al. 2001. Am J Hum Genet. 69: 989-1001. PMID: 11577371
3. Substitution of aspartate for glycine 1018 in the type III procollagen (COL3A1) gene causes type IV Ehlers-Danlos syndrome: the mutated allele is present in most blood leukocytes of the asymptomatic and mosaic mother.  |  Kontusaari, S., et al. 1992. Am J Hum Genet. 51: 497-507. PMID: 1496983
4. The procollagen type III, alpha 1 (COL3A1) gene first intron expresses poly-A+ RNA corresponding to multiple ESTs and putative miRNAs.  |  Sterling, KM. 2011. J Cell Biochem. 112: 541-7. PMID: 21268075
5. A base substitution at a splice site in the COL3A1 gene causes exon skipping and generates abnormal type III procollagen in a patient with Ehlers-Danlos syndrome type IV.  |  Cole, WG., et al. 1990. J Biol Chem. 265: 17070-7. PMID: 2145268
6. A mutation in the gene for type III procollagen (COL3A1) in a family with aortic aneurysms.  |  Kontusaari, S., et al. 1990. J Clin Invest. 86: 1465-73. PMID: 2243125
7. Inheritance of an RNA splicing mutation (G+ 1 IVS20) in the type III procollagen gene (COL3A1) in a family having aortic aneurysms and easy bruisability: phenotypic overlap between familial arterial aneurysms and Ehlers-Danlos syndrome type IV.  |  Kontusaari, S., et al. 1990. Am J Hum Genet. 47: 112-20. PMID: 2349939
8. Identical G+1 to A mutations in three different introns of the type III procollagen gene (COL3A1) produce different patterns of RNA splicing in three variants of Ehlers-Danlos syndrome. IV. An explanation for exon skipping some mutations and not others.  |  Kuivaniemi, H., et al. 1990. J Biol Chem. 265: 12067-74. PMID: 2365710
9. Cloning and analysis of the 5' portion of the human type-III procollagen gene (COL3A1).  |  Benson-Chanda, V., et al. 1989. Gene. 78: 255-65. PMID: 2777083
10. Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen.  |  Superti-Furga, A., et al. 1988. J Biol Chem. 263: 6226-32. PMID: 2834369

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pro-COL3A1 Antibody (A-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Pro-COL3A1 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pro-COL3A1 Antibody (A-1) Specifications

Alternate Product Name: Pro-COL3A1

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 30-54

Epitope Target: Procollagen α1 Type III

Epitope Species: human

Product Reactivity/Detection: Procollagen α1 Type III

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Pro-COL3A1 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred COL3A1 antibody is COL3A1 Antibody (B-10): sc-271249.