PRIP Antibody (E-12): sc-515547

PRIP Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRIP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRIP (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The peroxisome proliferator-activated receptor-interacting protein (PRIP), also known as nuclear receptor co-activator 6, plays a crucial role in cellular signaling by acting as a nuclear receptor co-activator. PRIP binds directly to nuclear receptors, enhancing their transcriptional activities in a hormone-dependent manner, which is vital for regulating gene expression in response to hormonal signals. PRIP is widely expressed across various tissues, with the highest levels found in the ovary, brain, testis, and prostate, indicating its importance in reproductive and neurological functions. Additionally, PRIP interacts with PRIP-interacting protein with methyltransferase activity (PIMT), facilitating communication between cAMP response element-binding protein-binding protein (CBP) and peroxisome proliferator-activated receptor gamma-binding protein-anchored (PBP) co-activator complexes, which are essential for the transcriptional regulation of nuclear receptors. Furthermore, PRIP is involved in modulating the phosphorylation of GABAA receptors by inhibiting the phosphatase PP1, thereby influencing synaptic inhibition and neuronal signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PRIP Antibody (E-12) References:

1. Isolation and characterization of peroxisome proliferator-activated receptor (PPAR) interacting protein (PRIP) as a coactivator for PPAR.  |  Zhu, Y., et al. 2000. J Biol Chem. 275: 13510-6. PMID: 10788465
2. Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function.  |  Zhu, Y., et al. 2001. Proc Natl Acad Sci U S A. 98: 10380-5. PMID: 11517327
3. Coactivator PRIP, the peroxisome proliferator-activated receptor-interacting protein, is a modulator of placental, cardiac, hepatic, and embryonic development.  |  Zhu, YJ., et al. 2003. J Biol Chem. 278: 1986-90. PMID: 12446700
4. [PRIP-1 involved in GABAA receptor trafficking].  |  Kanematsu, T. and Hirata, M. 2003. Seikagaku. 75: 378-82. PMID: 12822435
5. Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus.  |  Enünlü, I., et al. 2003. Biochem Biophys Res Commun. 309: 44-51. PMID: 12943661
6. Transcription coactivator peroxisome proliferator-activated receptor-binding protein/mediator 1 deficiency abrogates acetaminophen hepatotoxicity.  |  Jia, Y., et al. 2005. Proc Natl Acad Sci U S A. 102: 12531-6. PMID: 16109766
7. APP717, APP693, and PRIP gene mutations are rare in Alzheimer disease.  |  Schellenberg, GD., et al. 1991. Am J Hum Genet. 49: 511-7. PMID: 1679288
8. Protein phosphatase regulation by PRIP, a PLC-related catalytically inactive protein--implications in the phospho-modulation of the GABAA receptor.  |  Yanagihori, S., et al. 2006. Adv Enzyme Regul. 46: 203-22. PMID: 16854455
9. Thiamine-repressible expression vectors pREP and pRIP for fission yeast.  |  Maundrell, K. 1993. Gene. 123: 127-30. PMID: 8422996

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRIP Antibody (E-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PRIP Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRIP Antibody (E-12) Specifications

Alternate Product Name: PRIP

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1814-2045

Epitope Target: PRIP

Epitope Species: human

Product Reactivity/Detection: PRIP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PRIP Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin