PRIM1 Antibody (H-9): sc-390265
- PRIM1 Antibody (H-9) is a mouse monoclonal IgG1 κ PRIM1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 135-175 within an internal region of PRIM1 of human origin
- PRIM1 Antibody (H-9) is recommended for detection of PRIM1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-PRIM1 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PRIM1 Antibody (H-9) for WB applications. These reagents are now offered in bundles with PRIM1 Antibody (H-9) (see ordering information below).
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PRIM1 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRIM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRIM1 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRIM1 protein, also known as DNA primase small subunit or p49, plays a crucial role in DNA replication by synthesizing small RNA primers necessary for the formation of Okazaki fragments on the lagging strand. This 420 amino acid protein forms a heterodimer with PRIM2A, another essential component of the DNA primase complex, which is vital for the accurate and efficient replication of eukaryotic DNA. PRIM1′s importance extends beyond its fundamental role in DNA synthesis; PRIM1 is coamplified with other genes in the 12q13 amplicon in human osteosarcoma, suggesting a potential role in tumorigenesis. Additionally, studies in zebrafish have shown that PRIM1 mutations don′t affect cell proliferation but can trigger neuronal apoptosis, likely through the activation of the ATM-Chk2-p53 apoptotic pathway, highlighting PRIM1′s significance in cellular integrity and response to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRIM1 Antibody (H-9) References:
- Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma. | Yotov, WV., et al. 1999. Genes Chromosomes Cancer. 26: 62-9. PMID: 10441007
- Tissue factor enhances protease-activated receptor-2-mediated factor VIIa cell proliferative properties. | Fan, L., et al. 2005. J Thromb Haemost. 3: 1056-63. PMID: 15869604
- Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53. | Yamaguchi, M., et al. 2008. Development. 135: 1247-57. PMID: 18287205
- Physical mapping of the genes for three components of the mouse DNA replication complex: polymerase alpha to the X chromosome, primase p49 subunit to chromosome 10, and primase p58 subunit to chromosome 1. | Adler, DA., et al. 1991. Genomics. 9: 642-6. PMID: 2037291
- DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. | Stadlbauer, F., et al. 1994. Eur J Biochem. 222: 781-93. PMID: 8026492
- Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12. | Shiratori, A., et al. 1995. Genomics. 28: 350-3. PMID: 8530050
- Primase structure and function. | Griep, MA. 1995. Indian J Biochem Biophys. 32: 171-8. PMID: 8655184
- Mapping of the human DNA primase 1 (PRIM1) to chromosome 12q13. | Cloutier, S., et al. 1997. Genomics. 43: 398-401. PMID: 9268648
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRIM1 Antibody (H-9) | sc-390265 | 200 µg/ml | $322.00 | |||
PRIM1 Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-530114 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRIM1 Antibody (H-9): m-IgGκ BP-HRP Bundle | sc-523473 | 200 µg Ab, 40 µg BP | $361.00 | |||
PRIM1 Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-543876 | 200 µg Ab; 20 µg BP | $361.00 | |||
PRIM1 Antibody (H-9) AC | sc-390265 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PRIM1 Antibody (H-9) HRP | sc-390265 HRP | 200 µg/ml | $322.00 | |||
PRIM1 Antibody (H-9) FITC | sc-390265 FITC | 200 µg/ml | $336.00 | |||
PRIM1 Antibody (H-9) PE | sc-390265 PE | 200 µg/ml | $349.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 488 | sc-390265 AF488 | 200 µg/ml | $364.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 546 | sc-390265 AF546 | 200 µg/ml | $364.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 594 | sc-390265 AF594 | 200 µg/ml | $364.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 647 | sc-390265 AF647 | 200 µg/ml | $364.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 680 | sc-390265 AF680 | 200 µg/ml | $364.00 | |||
PRIM1 Antibody (H-9) Alexa Fluor® 790 | sc-390265 AF790 | 200 µg/ml | $364.00 | |||
PRIM1 (H-9) Neutralizing Peptide | sc-390265 P | 100 µg/0.5 ml | $69.00 |