PREP-2 Antibody (56.1): sc-101857

PREP-2 Antibody (56.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects PREP-2 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). PREP-2 (56.1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PREP-2, also known as Pbx-regulating protein-2 or PBX/knotted 1 homeobox 2, is a crucial member of the TALE (three amino acid loop extension)/MEIS family, playing a significant role in gene regulation. PREP-2 is primarily located in the nucleus, where PREP-2 forms stable complexes with Pbx proteins, facilitating the regulation of target genes essential for developmental processes. Nuclear localization of PREP-2 enables precise control of transcriptional activity, particularly in forming ternary complexes with Hox proteins, such as HoxB1. While PREP-1 enhances transcriptional activation within these complexes, PREP-2 exhibits a unique function by slightly reducing transcriptional activity, highlighting a distinct regulatory role. Multiple isoforms of PREP-2 exist, with some localizing to the cytoplasm, where PREP-2 interacts with cytoskeletal components like F-actin, G-actin, and tubulin/microtubules, further emphasizing PREP-2′s versatility and importance in cellular dynamics.

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PREP-2 Antibody (56.1) References:

1. Identification and characterization of human PKNOX2, a novel homeobox-containing gene.  |  Imoto, I., et al. 2001. Biochem Biophys Res Commun. 287: 270-6. PMID: 11549286
2. Characterization of PREP2, a paralog of PREP1, which defines a novel sub-family of the MEINOX TALE homeodomain transcription factors.  |  Fognani, C., et al. 2002. Nucleic Acids Res. 30: 2043-51. PMID: 11972344
3. Prep2: cloning and expression of a new prep family member.  |  Haller, K., et al. 2002. Dev Dyn. 225: 358-64. PMID: 12412021
4. Expression of Hox cofactor genes during mouse ovarian follicular development and oocyte maturation.  |  Villaescusa, JC., et al. 2004. Gene. 330: 1-7. PMID: 15087118
5. Subcellular localization of multiple PREP2 isoforms is regulated by actin, tubulin, and nuclear export.  |  Haller, K., et al. 2004. J Biol Chem. 279: 49384-94. PMID: 15339927
6. Hydrolethalus syndrome is caused by a missense mutation in a novel gene HYLS1.  |  Mee, L., et al. 2005. Hum Mol Genet. 14: 1475-88. PMID: 15843405
7. The HOXC13-controlled expression of early hair keratin genes in the human hair follicle does not involve TALE proteins MEIS and PREP as cofactors.  |  Jave-Suárez, LF. and Schweizer, J. 2006. Arch Dermatol Res. 297: 372-6. PMID: 16292560
8. Toward understanding the genetics of alcohol drinking through transcriptome meta-analysis.  |  Mulligan, MK., et al. 2006. Proc Natl Acad Sci U S A. 103: 6368-73. PMID: 16618939

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PREP-2 Antibody (56.1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PREP-2 Antibody (56.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PREP-2 Antibody (56.1) Specifications

Alternate Product Name: PREP-2

Product Clone Name: 56.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 90-435

Epitope Target: PREP-2

Epitope Species: human

Product Reactivity/Detection: PREP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PREP-2 Antibody (56.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin