PREP-1 Antibody (B-2): sc-25282

PREP-1 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects PREP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PREP-1 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PREP-1 plays a crucial role in transcriptional regulation, particularly in pre-B cell acute leukemias, where PREP-1 is often implicated in chromosomal rearrangements such as the t(1;19)(q23;p13.3) that leads to the formation of a fusion gene between E2A and Pbx1. This fusion results in a protein that combines the transactivation domain of E2A with the homeobox domain of Pbx1, which is essential for the regulation of genes involved in cell proliferation and differentiation. Additionally, PREP-1 interacts with Pbx proteins to form stable complexes that enhance the transcription of the urokinase gene, thereby influencing processes such as cell migration and invasion. PREP-1′s ability to modulate transcriptional activity makes PREP-1 a significant factor in both normal cellular functions and pathological conditions, highlighting PREP-1′s importance in cancer research and therapeutic development.

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PREP-1 Antibody (B-2) References:

1. PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1.  |  Monica, K., et al. 1991. Mol Cell Biol. 11: 6149-57. PMID: 1682799
2. Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor.  |  Nourse, J., et al. 1990. Cell. 60: 535-45. PMID: 1967982
3. A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL.  |  Kamps, MP., et al. 1990. Cell. 60: 547-55. PMID: 1967983
4. Pknox1/Prep1 regulates mitochondrial oxidative phosphorylation components in skeletal muscle.  |  Kanzleiter, T., et al. 2014. Mol Cell Biol. 34: 290-8. PMID: 24216763
5. Prep1 deficiency improves metabolic response in white adipose tissue.  |  Liotti, A., et al. 2018. Biochim Biophys Acta Mol Cell Biol Lipids. 1863: 515-525. PMID: 29474930
6. Prep1, A Homeodomain Transcription Factor Involved in Glucose and Lipid Metabolism.  |  Oriente, F., et al. 2018. Front Endocrinol (Lausanne). 9: 346. PMID: 30002646
7. The genetics and the molecular functions of the PREP1 homeodomain transcription factor.  |  Purushothaman, D. and Blasi, F. 2018. Int J Dev Biol. 62: 819-825. PMID: 30604851
8. Prep1 regulates angiogenesis through a PGC-1α-mediated mechanism.  |  Cimmino, I., et al. 2019. FASEB J. 33: 13893-13904. PMID: 31618597
9. Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation.  |  Lu, Q., et al. 1994. Mol Cell Biol. 14: 3938-48. PMID: 7910944
10. Transformation properties of the E2a-Pbx1 chimeric oncoprotein: fusion with E2a is essential, but the Pbx1 homeodomain is dispensable.  |  Monica, K., et al. 1994. Mol Cell Biol. 14: 8304-14. PMID: 7969166
11. Fusion with E2A alters the transcriptional properties of the homeodomain protein PBX1 in t(1;19) leukemias.  |  LeBrun, DP. and Cleary, ML. 1994. Oncogene. 9: 1641-7. PMID: 8183558
12. Purification and characterization of UEF3, a novel factor involved in the regulation of the urokinase and other AP-1 controlled promoters.  |  Berthelsen, J., et al. 1996. J Biol Chem. 271: 3822-30. PMID: 8632000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PREP-1 Antibody (B-2) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-PREP-1 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PREP-1 Antibody (B-2) Specifications

Alternate Product Name: PREP-1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 15-436

Epitope Target: PREP-1

Epitope Species: human

Product Reactivity/Detection: PREP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for PREP-1 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent