PR Antibody (A-2) recommended for detection of PR-A and PR-B of mouse, rat and human origin by WB, IP, IF and ELISA

PR Antibody (A-2): sc-398898

PR Antibody (A-2) is rated 4.0 out of 5 by 4.
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Datasheets

    • PR Antibody (A-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 538-571 within an internal region of PR of human origin
    • Anti-PR Antibody (A-2) is recommended for detection of PR-A and PR-B of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-PR Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-398898 X, 200 µg/0.1 ml)
    • blocking peptide, sc-398898 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PR (A-2): sc-398898.
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What application is the blocking peptide sc-398898 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Ishikawa細胞ではいまひとつです。 Ishikawa細胞のタンパクを10μg applyしました。あまりきれいにバンドが出ませんでした。
Date published: 2018-02-23
Rated 5 out of 5 by from PR Antibody (A-2) works well with mouse breast cancer tissue, strong and specific signal without background.
Date published: 2018-01-05
Rated 5 out of 5 by from Good WB results This monoclonal PR Antibody (A-2) gives good results in Western blotting in A-431, MCF7 and AT3B-1 whole cell lysates.
Date published: 2017-02-07
Rated 5 out of 5 by from Very good Western blot data of PR expression in T Very good Western blot data of PR expression in T-47D whole cell lysate. -SCBT QC
Date published: 2014-04-19
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