PPIH Antibody (C-5): sc-390798

PPIH Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects PPIH (peptidylprolyl isomerase H) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PPIH is a 177 amino acid protein that plays a crucial role in protein folding and catalyzes the cis-trans isomerization of proline imidic peptide bonds, which is essential for the proper functioning of numerous proteins. Anti-PPIH antibody (C-5) recognizes a protein particularly important in pre-mRNA splicing, where PPIH interacts with processing factors such as PRPF3, PRPF4, and PRPF18, contributing to the assembly of the U4/U5/U6 tri-snRNP complex. As a protein chaperone, PPIH not only facilitates the correct folding of proteins but also mediates interactions within the spliceosome, ensuring efficient splicing and gene expression regulation. The presence of a cyclophilin-type domain in PPIH allows PPIH to exhibit peptidyl-prolyl isomerase activity, which is inhibited by cyclosporin A, highlighting PPIH′s potential as a target for therapeutic intervention in diseases where protein misfolding is a factor. PPIH monoclonal antibody (C-5) serves as an invaluable tool for researchers studying the intricate processes of RNA splicing and protein interactions in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PPIH Antibody (C-5) References:

1. The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors.  |  Pfefferle, S., et al. 2011. PLoS Pathog. 7: e1002331. PMID: 22046132
2. Identification and taste characteristics of novel umami and umami-enhancing peptides separated from peanut protein isolate hydrolysate by consecutive chromatography and UPLC-ESI-QTOF-MS/MS.  |  Zhang, J., et al. 2019. Food Chem. 278: 674-682. PMID: 30583429
3. The Perilous PPI: Proton Pump Inhibitor as a Cause of Clinically Significant Hypomagnesaemia.  |  Tai, YT. and Tong, CV. 2020. J ASEAN Fed Endocr Soc. 35: 109-113. PMID: 33442177
4. Systematic Analysis Identifies a Specific RNA-Binding Protein-Related Gene Model for Prognostication and Risk-Adjustment in HBV-Related Hepatocellular Carcinoma.  |  Li, M., et al. 2021. Front Genet. 12: 707305. PMID: 34422009
5. Identification of the susceptibility genes for COVID-19 in lung adenocarcinoma with global data and biological computation methods.  |  Gao, L., et al. 2021. Comput Struct Biotechnol J. 19: 6229-6239. PMID: 34840672
6. Exploring immunoregulatory properties of a phenolic-enriched maple syrup extract through integrated proteomics and in vitro assays.  |  Puopolo, T., et al. 2024. Food Funct. 15: 172-182. PMID: 38019191
7. A Case of Severe Proton Pump Inhibitor-Induced Hypomagnesemia Refractory to Continuous Oral and Intravenous Magnesium Replenishment.  |  Kheir, K., et al. 2024. Cureus. 16: e54483. PMID: 38516446
8. System analysis based on T-cell exhaustion-related genes identifies PTPRT as a promising diagnostic and prognostic biomarker for gastric cancer.  |  Wu, J., et al. 2024. Sci Rep. 14: 21049. PMID: 39251810
9. Doppler ultrasound of the uterine arteries: the importance of bilateral notching in the prediction of pre-eclampsia, placental abruption or delivery of a small-for-gestational-age baby.  |  Harrington, K., et al. 1996. Ultrasound Obstet Gynecol. 7: 182-8. PMID: 8705410
10. Doppler assessment of the maternal interlobar renal and uterine arteries in mid-pregnancy in women at low and high risk for pregnancy-induced hypertension.  |  Zimmermann, P. and Ranta, T. 1998. J Clin Ultrasound. 26: 239-45. PMID: 9608366

The preferred PPIH antibody is PPIH Antibody (C-1): sc-377217.