PPIH Antibody (C-1): sc-377217

PPIH Antibody (C-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects PPIH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PPIH antibody (C-1) is available as the non-conjugated form. PPIH, or peptidylprolyl isomerase H, is a 177 amino acid protein that plays a crucial role in protein folding and isomerization, specifically catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This function is vital as PPIH facilitates proper folding of proteins, which is essential for their stability and functionality. Additionally, PPIH is implicated in pre-mRNA splicing, interacting with processing factors such as PRPF3, PRPF4, and PRPF18, and contributes to the assembly of the U4/U5/U6 tri-snRNP complex, a critical component of the spliceosome. As a protein chaperone, PPIH not only possesses peptidylprolyl isomerase activity but also mediates interactions between various proteins within the spliceosome, highlighting its importance in the regulation of gene expression. PPIH contains one PPIase cyclophilin-type domain and is inhibited by cyclosporin A, making PPIH a significant target for studies related to protein interactions and splicing mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PPIH Antibody (C-1) References:

1. Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide.  |  Reidt, U., et al. 2003. J Mol Biol. 331: 45-56. PMID: 12875835
2. Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH.  |  Ingelfinger, D., et al. 2003. Nucleic Acids Res. 31: 4791-6. PMID: 12907720
3. Function-dependent clustering of orthologues and paralogues of cyclophilins.  |  Galat, A. 2004. Proteins. 56: 808-20. PMID: 15281132
4. Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex.  |  Gonzalez-Santos, JM., et al. 2008. Hum Mol Genet. 17: 225-39. PMID: 17932117
5. The assembly of a spliceosomal small nuclear ribonucleoprotein particle.  |  Patel, SB. and Bellini, M. 2008. Nucleic Acids Res. 36: 6482-93. PMID: 18854356
6. PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis.  |  Ye, J., et al. 2023. Aging (Albany NY). 15: 11448-11470. PMID: 37874737
7. PPIH acts as a potential predictive biomarker for patients with common solid tumors.  |  Ye, J., et al. 2024. BMC Cancer. 24: 681. PMID: 38834966
8. PPIH is a novel diagnostic biomarker associated with immune infiltration in cholangiocarcinoma.  |  Ye, J., et al. 2025. BMC Cancer. 25: 218. PMID: 39920663
9. Overexpression of p59-HBI (FKBP59), full length and domains, and characterization of PPlase activity.  |  Chambraud, B., et al. 1993. Biochem Biophys Res Commun. 196: 160-6. PMID: 8216288
10. A cyclophilin-like peptidyl-prolyl cis/trans isomerase from Legionella pneumophila--characterization, molecular cloning and overexpression.  |  Schmidt, B., et al. 1996. Mol Microbiol. 21: 1147-60. PMID: 8898384
11. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs.  |  Horowitz, DS., et al. 1997. RNA. 3: 1374-87. PMID: 9404889
12. The 20kD protein of human [U4/U6.U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60kD and 90kD proteins.  |  Teigelkamp, S., et al. 1998. RNA. 4: 127-41. PMID: 9570313

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PPIH Antibody (C-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PPIH Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PPIH Antibody (C-1) Specifications

Alternate Product Name: PPIH

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 75-107

Epitope Target: PPIH

Epitope Species: human

Product Reactivity/Detection: PPIH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PPIH Antibody (C-1): P: Blocking Peptide, X: TransCruz Reagent