PPARγ₂ Antibody (A-1): sc-166731

PPARγ2 Antibody (A-1) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect the peroxisome proliferator-activated receptor gamma 2 (PPARγ2) protein by targeting an epitope within amino acids 2-20 at the N-terminus of human PPARγ2. Anti-PPARγ2 antibody (A-1) is indispensable for researchers studying the intricate functions of PPARγ2, a pivotal nuclear hormone receptor that plays a critical role in regulating adipocyte differentiation, lipid metabolism, insulin sensitivity, and inflammatory responses. PPARγ2, also known as Nr1c3, PPARgamma, and PPARgamma2, operates by forming heterodimers with retinoid X receptors (RXRs), which then bind to specific DNA sequences to modulate the transcription of genes involved in these essential metabolic processes. This regulation is crucial for maintaining energy homeostasis and is implicated in the pathogenesis of various metabolic diseases, including obesity, type 2 diabetes, atherosclerosis, and certain cancers. PPARγ2 monoclonal antibody (A-1) offers exceptional specificity by exclusively recognizing PPARγ2 without cross-reacting with the closely related PPARγ1 isoform, enabling precise differentiation and study of PPARγ2′s unique functions. PPARγ2 (A-1) antibody is validated for multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), ensuring versatility and reliability in various experimental setups. Available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and several Alexa Fluor® variants, PPARγ2 (A-1) monoclonal antibody provides researchers with the flexibility to tailor their experiments to specific detection and visualization needs. Anti-PPARγ2 antibody (A-1) supports critical investigations into the molecular mechanisms governing metabolic regulation, the development of therapeutic strategies for related diseases, and the broader understanding of PPARγ2′s role in cellular processes.

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PPARγ₂ Antibody (A-1) References:

1. β-catenin directly sequesters adipocytic and insulin sensitizing activities but not osteoblastic activity of PPARγ2 in marrow mesenchymal stem cells.  |  Rahman, S., et al. 2012. PLoS One. 7: e51746. PMID: 23272157
2. Transient expression, purification and characterisation of human full-length PPARγ2 in HEK293 cells.  |  Liu, J., et al. 2013. Protein Expr Purif. 89: 189-95. PMID: 23562662
3. A novel PPARγ2 modulator sLZIP controls the balance between adipogenesis and osteogenesis during mesenchymal stem cell differentiation.  |  Kim, J. and Ko, J. 2014. Cell Death Differ. 21: 1642-55. PMID: 24948012
4. Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA.  |  Wan, R., et al. 2019. Diabetes Metab Syndr Obes. 12: 2273-2279. PMID: 31802926
5. Regulation of PPARγ2 Stability and Activity by SHP-1.  |  Kumar, A., et al. 2024. Mol Cell Biol. 44: 261-272. PMID: 38828991
6. Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat.  |  Braissant, O., et al. 1996. Endocrinology. 137: 354-66. PMID: 8536636
7. Expression of the peroxisome proliferator-activated receptor alpha gene is stimulated by stress and follows a diurnal rhythm.  |  Lemberger, T., et al. 1996. J Biol Chem. 271: 1764-9. PMID: 8576180
8. Differential activation of adipogenesis by multiple PPAR isoforms.  |  Brun, RP., et al. 1996. Genes Dev. 10: 974-84. PMID: 8608944
9. Regulation of peroxisome proliferator-activated receptor-alpha mRNA in rat liver.  |  Sterchele, PF., et al. 1996. Arch Biochem Biophys. 326: 281-9. PMID: 8611035
10. The orphan nuclear hormone receptor LXR alpha interacts with the peroxisome proliferator-activated receptor and inhibits peroxisome proliferator signaling.  |  Miyata, KS., et al. 1996. J Biol Chem. 271: 9189-92. PMID: 8621574
11. Crosstalk between the thyroid hormone and peroxisome proliferator-activated receptors in regulating peroxisome proliferator-responsive genes.  |  Hunter, J., et al. 1996. Mol Cell Endocrinol. 116: 213-21. PMID: 8647322
12. Expression of the peroxisome proliferator-activated receptor (PPAR) in the mouse colonic mucosa.  |  Mansén, A., et al. 1996. Biochem Biophys Res Commun. 222: 844-51. PMID: 8651933

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PPARγ₂ Antibody (A-1) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-PPARγ₂ Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PPARγ₂ Antibody (A-1) Specifications

Alternate Product Name: PPAR gamma 2

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2-20

Epitope Target: PPARγ₂

Epitope Species: human

Product Reactivity/Detection: PPARγ₂

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for PPARγ₂ Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred PPARγ antibody is PPARγ Antibody (E-8): sc-7273.