PP2A-Aα/β Antibody (A-5): sc-74580
- PP2A-Aα/β Antibody (A-5) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 290-589 mapping at the C-terminus of PP2A-Aα of human origin
- recommended for detection of PP2A-Aα and PP2A-Aβ of mouse, rat and human origin by WB, IP, IF and ELISA
- See PP2A-Aα (6G3): sc-56954 for PP2A-Aα/β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP is the preferred secondary detection reagent for PP2A-Aα/β Antibody (A-5) for WB applications. This reagent is now offered in a bundle with PP2A-Aα/β Antibody (A-5) (see ordering information below).
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PP2A-Aα/β Antibody (A-5) is a mouse monoclonal IgG2b antibody that detects PP2A-Aα/β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PP2A-Aα/β plays a crucial role in cellular signaling pathways by acting as a regulatory subunit of the protein phosphatase 2A (PP2A) complex, which is essential for the dephosphorylation of serine and threonine residues on target proteins. This dephosphorylation is vital for various cellular processes, including cell division, apoptosis, and maintaining cellular homeostasis. The PP2A complex is composed of a catalytic subunit and regulatory subunits, with PP2A-Aα and PP2A-Aβ being key members that modulate the activity and specificity of the phosphatase. The interaction of PP2A-Aα/β with different regulatory subunits allows for the fine-tuning of signaling pathways, making PP2A-Aα/β a significant player in the regulation of cell growth and differentiation. Understanding PP2A-Aα/β dynamics and interactions is important for elucidating the mechanisms underlying various diseases, including cancer, where dysregulation of phosphatase activity can lead to uncontrolled cell proliferation.
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PP2A-Aα/β Antibody (A-5) References:
- SV40 small T antigen and PP2A phosphatase in cell transformation. | Sablina, AA. and Hahn, WC. 2008. Cancer Metastasis Rev. 27: 137-46. PMID: 18214640
- The Basic Biology of PP2A in Hematologic Cells and Malignancies. | Haesen, D., et al. 2014. Front Oncol. 4: 347. PMID: 25566494
- PP2A as a master regulator of the cell cycle. | Wlodarchak, N. and Xing, Y. 2016. Crit Rev Biochem Mol Biol. 51: 162-84. PMID: 26906453
- Roles and regulation of protein phosphatase 2A (PP2A) in the heart. | Lubbers, ER. and Mohler, PJ. 2016. J Mol Cell Cardiol. 101: 127-133. PMID: 27832939
- Non-genomic mechanisms of protein phosphatase 2A (PP2A) regulation in cancer. | Kauko, O. and Westermarck, J. 2018. Int J Biochem Cell Biol. 96: 157-164. PMID: 29355757
- PP2A holoenzymes, substrate specificity driving cellular functions and deregulation in cancer. | Fowle, H., et al. 2019. Adv Cancer Res. 144: 55-93. PMID: 31349904
- A Cancer-Associated Missense Mutation in PP2A-Aα Increases Centrosome Clustering during Mitosis. | Antao, NV., et al. 2019. iScience. 19: 74-82. PMID: 31357169
- Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α. | Tang, Y., et al. 2019. Biomed Res Int. 2019: 2582401. PMID: 31641668
- Critical Functions of PP2A-Like Protein Phosphotases in Regulating Meiotic Progression. | Lei, WL., et al. 2021. Front Cell Dev Biol. 9: 638559. PMID: 33718377
- Novel PP2A-Activating Compounds in Neuroblastoma. | Nazam, N., et al. 2024. Cancers (Basel). 16: PMID: 39594793
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP2A-Aα/β Antibody (A-5) | sc-74580 | 200 µg/ml | $322.00 | |||
PP2A-Aα/β Antibody (A-5): m-IgG2b BP-HRP Bundle | sc-548464 | 200 µg Ab; 10 µg BP | $361.00 |