PP2A-Aα Antibody (6G3): sc-56954

PP2A-Aα Antibody (6G3) is a rat monoclonal IgG2a antibody that detects PP2A-A alpha protein of mouse, rat, human, Xenopus, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-PP2A-Aα antibody (6G3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-A alpha protein plays a crucial role in cellular signaling by acting as a serine/threonine phosphatase, which is essential for the regulation of numerous cellular processes such as cell division, differentiation, and apoptosis. PP2A-A alpha′s ability to dephosphorylate target proteins allows modulation of various signaling pathways, making PP2A-A alpha a key player in maintaining cellular homeostasis and responding to stress signals. The structural integrity of the PP2A complex, which consists of a regulatory subunit, a variable subunit, and a catalytic subunit, is vital for function, as any disruption can lead to dysregulation of these critical pathways and has been implicated in various diseases, including cancer and neurodegenerative disorders. Anti-PP2A-Aα antibody (6G3) enables researchers to gain valuable insights into the dynamics of protein phosphorylation and its implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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PP2A-Aα Antibody (6G3) References:

1. Low frequency of alterations of the alpha (PPP2R1A) and beta (PPP2R1B) isoforms of the subunit A of the serine-threonine phosphatase 2A in human neoplasms.  |  Calin, GA., et al. 2000. Oncogene. 19: 1191-5. PMID: 10713707
2. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
3. PPP2R1A mutation is a rare event in ovarian carcinoma across histological subtypes.  |  Rahman, M., et al. 2013. Anticancer Res. 33: 113-8. PMID: 23267135
4. PPP2R1A regulated by PAX3/FOXO1 fusion contributes to the acquisition of aggressive behavior in PAX3/FOXO1-positive alveolar rhabdomyosarcoma.  |  Akaike, K., et al. 2018. Oncotarget. 9: 25206-25215. PMID: 29861864
5. Refractory Epilepsy in a Toddler With PPP2R1A Gene Mutation and Congenital Hydrocephalus.  |  Ruxmohan, S., et al. 2021. Cureus. 13: e19988. PMID: 34984142
6. Quantum-based modeling implies that bidentate Arg89-substrate binding enhances serine/threonine protein phosphatase-2A(PPP2R5D/PPP2R1A/PPP2CA)-mediated dephosphorylation.  |  Salter, EA., et al. 2023. Front Cell Dev Biol. 11: 1141804. PMID: 37377738
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-Aα Antibody (6G3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-Aα Antibody (6G3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-Aα Antibody (6G3) Specifications

Alternate Product Name: PP2A phosphatase structural subunit A alpha

Product Clone Name: 6G3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: PP2A-Aα

Epitope Species: human

Product Reactivity/Detection: PP2A-Aα

Species Reactivity/Detection: mouse, rat, human, Xenopus, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for PP2A-Aα Antibody (6G3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PP2A-Aα/β antibody is PP2A-Aα/β Antibody (4G7): sc-13600.