Positive cofactor 4 Antibody (H-12): sc-166280

Positive cofactor 4 Antibody (H-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Positive cofactor 4 (PC4) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PC4 Antibody (H-12) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PC4 plays a crucial role in transcription regulation within eukaryotic cells as part of high molecular weight coactivating complexes that facilitate communication between transcriptional activators and RNA polymerase II (RNAPII). PC4 interacts with transcription factor IIA (TFIIA) and TATA-binding protein-associated factors (TAFs), enabling direct binding to double-stranded DNA. This interaction is essential for activating and repressing RNAPII-mediated transcription, varying with specific transcription factors and holoenzyme components. PC4 undergoes phosphorylation by TFIID and TFIIH, leading to PC4 release from the DNA promoter region, inhibiting assembly into the transcriptional promoting complex and blocking transcription. Such dynamic regulation underscores PC4′s importance in gene expression and cellular function, making PC4 a valuable target for research in transcriptional control mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Positive cofactor 4 Antibody (H-12) References:

1. Sub1/PC4 a chromatin associated protein with multiple functions in transcription.  |  Conesa, C. and Acker, J. 2010. RNA Biol. 7: 287-90. PMID: 20305379
2. Inhibition of human positive cofactor 4 radiosensitizes human esophageal squmaous cell carcinoma cells by suppressing XLF-mediated nonhomologous end joining.  |  Qian, D., et al. 2014. Cell Death Dis. 5: e1461. PMID: 25321468
3. An integrated systems biology approach identifies positive cofactor 4 as a factor that increases reprogramming efficiency.  |  Jo, J., et al. 2016. Nucleic Acids Res. 44: 1203-15. PMID: 26740582
4. Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.  |  Pan, Q., et al. 2024. J Cell Biol. 223: PMID: 38349334
5. PC4 promotes bladder cancer progression and stemness by directly interacting with Sp1 to transcriptionally activate the Wnt5a/β-catenin pathway.  |  Yan, B., et al. 2024. Pathol Res Pract. 259: 155369. PMID: 38820928
6. The coactivator p15 (PC4) initiates transcriptional activation during TFIIA-TFIID-promoter complex formation.  |  Kaiser, K., et al. 1995. EMBO J. 14: 3520-7. PMID: 7628453
7. Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes.  |  Ge, H. and Roeder, RG. 1994. Cell. 78: 513-23. PMID: 8062391
8. A mammalian SRB protein associated with an RNA polymerase II holoenzyme.  |  Chao, DM., et al. 1996. Nature. 380: 82-5. PMID: 8598913
9. A dynamic model for PC4 coactivator function in RNA polymerase II transcription.  |  Malik, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2192-7. PMID: 9482861
10. Properties of PC4 and an RNA polymerase II complex in directing activated and basal transcription in vitro.  |  Wu, SY. and Chiang, CM. 1998. J Biol Chem. 273: 12492-8. PMID: 9575207
11. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
12. TAFII-independent activation mediated by human TBP in the presence of the positive cofactor PC4.  |  Wu, SY., et al. 1998. EMBO J. 17: 4478-90. PMID: 9687514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Positive cofactor 4 Antibody (H-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Positive cofactor 4 Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Positive cofactor 4 Antibody (H-12) Specifications

Alternate Product Name: Positive cofactor 4

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 14-127

Epitope Target: Positive cofactor 4

Epitope Species: human

Product Reactivity/Detection: Positive cofactor 4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Positive cofactor 4 Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent