POPX2 Antibody (E-2): sc-514894
- POPX2 Antibody (E-2) is a mouse monoclonal IgG2a κ POPX2 antibody provided at 200 µg/ml
- raised against amino acids 1-100 mapping at the N-terminus of POPX2 of mouse origin
- POPX2 Antibody (E-2) is recommended for detection of POPX2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-POPX2 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for POPX2 Antibody (E-2) for WB applications. These reagents are now offered in bundles with POPX2 Antibody (E-2) (see ordering information below).
QUICK LINKS
POPX2 Antibody (E-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects POPX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POPX2 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POPX2 plays a crucial role in cellular signaling by acting as a serine/threonine phosphatase, which is essential for the regulation of numerous cellular functions such as cell division, homeostasis, and apoptosis. As a member of the PP2C family of phosphatases, POPX2 is involved in the negative regulation of cell stress response pathways, thereby maintaining cellular integrity under stress conditions. POPX2 shows ubiquitous expression, allowing participation in various signaling pathways across different tissues. Notably, POPX2 interacts with PIX proteins, which are critical for modulating the activity of αPAK, a protein kinase that influences actin dynamics and cellular morphology. Additionally, POPX2 regulates the activity of CaMKII, further underscoring POPX2′s importance in calcium signaling and neuronal function. Dysregulation of POPX2 expression can lead to significant cellular consequences, including caspase-dependent apoptosis, highlighting POPX2′s potential role in various pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POPX2 Antibody (E-2) References:
- Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Kikuno, R., et al. 1999. DNA Res. 6: 197-205. PMID: 10470851
- The Caenorhabditis elegans sex-determining protein FEM-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis. | Tan, KM., et al. 2001. J Biol Chem. 276: 44193-202. PMID: 11559703
- The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. | Koh, CG., et al. 2002. Curr Biol. 12: 317-21. PMID: 11864573
- Regulation of the multifunctional Ca2+/calmodulin-dependent protein kinase II by the PP2C phosphatase PPM1F in fibroblasts. | Harvey, BP., et al. 2004. J Biol Chem. 279: 24889-98. PMID: 15140879
- Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. | Ishida, A., et al. 2005. Arch Biochem Biophys. 435: 134-46. PMID: 15680915
- Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1. | Nomura, N., et al. 1994. DNA Res. 1: 27-35. PMID: 7584026
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POPX2 Antibody (E-2) | sc-514894 | 200 µg/ml | $316.00 | |||
POPX2 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531241 | 200 µg Ab; 10 µg BP | $354.00 | |||
POPX2 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-524873 | 200 µg Ab, 40 µg BP | $354.00 | |||
POPX2 Antibody (E-2): m-IgG2a BP-HRP Bundle | sc-547738 | 200 µg Ab; 10 µg BP | $354.00 | |||
POPX2 Antibody (E-2) AC | sc-514894 AC | 500 µg/ml, 25% agarose | $416.00 | |||
POPX2 Antibody (E-2) HRP | sc-514894 HRP | 200 µg/ml | $316.00 | |||
POPX2 Antibody (E-2) FITC | sc-514894 FITC | 200 µg/ml | $330.00 | |||
POPX2 Antibody (E-2) PE | sc-514894 PE | 200 µg/ml | $343.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 488 | sc-514894 AF488 | 200 µg/ml | $357.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 546 | sc-514894 AF546 | 200 µg/ml | $357.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 594 | sc-514894 AF594 | 200 µg/ml | $357.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 647 | sc-514894 AF647 | 200 µg/ml | $357.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 680 | sc-514894 AF680 | 200 µg/ml | $357.00 | |||
POPX2 Antibody (E-2) Alexa Fluor® 790 | sc-514894 AF790 | 200 µg/ml | $357.00 |