Pontin 52 Antibody (A-4): sc-393854
- Pontin 52 Antibody (A-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 191-290 mapping within an internal region of Pontin 52 of human origin
- recommended for detection of Pontin 52 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- TransCruz reagent for ChIP application (sc-393854 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pontin 52 Antibody (A-4) for WB applications. These reagents are now offered in bundles with Pontin 52 Antibody (A-4) (see ordering information below).
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Pontin 52 Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects Pontin 52 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pontin 52, also known as RUVBL1 or NMP 238, is a crucial nuclear matrix protein primarily localized in the nucleoplasm of cells, while notably absent from nucleoli. This localization suggests a role in gene expression regulation and cellular processes within the nucleus. Pontin 52 features an ATPase/helicase motif, indicating involvement in various molecular pathways, particularly in transcriptional regulation. Pontin 52 forms complexes with other proteins, such as Myc and Reptin 52 (RUVBL2), highlighting importance in oncogenic processes. The interaction between Pontin 52 and Myc relies on a specific Myc domain essential for oncogenic activity, positioning Pontin 52 as a vital mediator in Myc-driven transformation. The gene encoding human Pontin 52 is located on chromosome 3q21, emphasizing relevance in cellular function and potential implications in cancer biology.
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Pontin 52 Antibody (A-4) References:
- Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity. | Bauer, A., et al. 2000. EMBO J. 19: 6121-30. PMID: 11080158
- Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a. | Carlson, ML., et al. 2003. Mol Cancer. 2: 42. PMID: 14675489
- AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. | Izumi, N., et al. 2010. Sci Signal. 3: ra27. PMID: 20371770
- RuvbL1 and RuvbL2 enhance aggresome formation and disaggregate amyloid fibrils. | Zaarur, N., et al. 2015. EMBO J. 34: 2363-82. PMID: 26303906
- RuvBL1 Maintains Resistance to TRAIL-Induced Apoptosis by Suppressing c-Jun/AP-1 Activity in Non-Small Cell Lung Cancer. | Li, H., et al. 2021. Front Oncol. 11: 679243. PMID: 34164343
- The Expression of the RUVBL1 Component of the R2TP Complex Correlates with Poor Prognosis in DLBCL. | Lone, M., et al. 2022. Pathobiology. 89: 146-156. PMID: 35078195
- Involvement of RUVBL1 in WNT/β-Catenin Signaling in Oral Squamous Cell Carcinoma. | Zeng, Y., et al. 2022. Dis Markers. 2022: 3398492. PMID: 35493294
- High expression of RUVBL1 and HNRNPU is associated with poor overall survival in stage I and II non-small cell lung cancer patients. | Durślewicz, J., et al. 2022. Discov Oncol. 13: 106. PMID: 36242708
- Downregulation of AHNAK2 inhibits cell cycle of lung adenocarcinoma cells by interacting with RUVBL1. | Li, X., et al. 2023. Thorac Cancer. 14: 2093-2104. PMID: 37349884
- RUVBL1 in Clear-Cell Renal Cell Carcinoma: Unraveling Prognostic Significance and Correlation with HIF1A. | Durślewicz, J., et al. 2024. Cancers (Basel). 16: PMID: 38610951
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pontin 52 Antibody (A-4) | sc-393854 | 200 µg/ml | $322.00 | |||
Pontin 52 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-538325 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pontin 52 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-535909 | 200 µg Ab; 40 µg BP | $361.00 | |||
Pontin 52 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-545643 | 200 µg Ab; 20 µg BP | $361.00 | |||
Pontin 52 Antibody (A-4) X | sc-393854 X | 200 µg/0.1 ml | $322.00 |