Pol III RPC32 Antibody (C32-1): sc-21754
- Pol III RPC32 Antibody (C32-1) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
- raised against recombinant human RPC32 subunit of RNA polymerase III
- Anti-Pol III RPC32 Antibody (C32-1) is recommended for detection of RPC 32 subunit of RNA polymerase III of mouse, rat and human origin by WB, IP and IF
- Anti-Pol III RPC32 Antibody (C32-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Pol III RPC32 Antibody (C32-1) for WB applications. This reagent is now offered in a bundle with Pol III RPC32 Antibody (C32-1) (see ordering information below).
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Pol III RPC32 Antibody (C32-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the RPC 32 subunit of RNA polymerase III in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-RPC32 antibody (C32-1) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The RPC 32 subunit plays a crucial role in the assembly and function of RNA polymerase III, which is responsible for synthesizing essential RNA molecules, including transfer RNA and 5S ribosomal RNA. These RNA types are vital for protein synthesis and cellular function, as they facilitate the translation of genetic information into functional proteins. Proper functioning of RNA polymerase III is essential for maintaining cellular homeostasis and responding to various physiological demands. Disruptions in RNA polymerase III activity can lead to significant cellular dysfunction and are implicated in various diseases, making the study of RPC 32 and its interactions critical for understanding gene expression regulation and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pol III RPC32 Antibody (C32-1) References:
- General transcription factors and subunits of RNA polymerase III: Paralogs for promoter- and cell type-specific transcription in multicellular eukaryotes. | Teichmann, M., et al. 2010. Transcription. 1: 130-135. PMID: 21326886
- Structural analysis of human RPC32β-RPC62 complex. | Boissier, F., et al. 2015. J Struct Biol. 192: 313-319. PMID: 26394183
- Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression. | Khattar, E., et al. 2016. J Clin Invest. 126: 4045-4060. PMID: 27643433
- Retrotransposon targeting to RNA polymerase III-transcribed genes. | Cheung, S., et al. 2018. Mob DNA. 9: 14. PMID: 29713390
- RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. | Liu, S., et al. 2021. Cell. 184: 1314-1329.e10. PMID: 33626331
- RNA Polymerase III Subunit Mutations in Genetic Diseases. | Lata, E., et al. 2021. Front Mol Biosci. 8: 696438. PMID: 34395528
- RNA polymerase III directly participates in DNA homologous recombination. | Liu, S., et al. 2022. Trends Cell Biol. 32: 988-995. PMID: 35811227
- RNA polymerase III transcription and cancer: A tale of two RPC7 subunits. | Cheng, R. and Van Bortle, K. 2022. Front Mol Biosci. 9: 1073795. PMID: 36710885
- A Combinatorial Regulatory Platform Determines Expression of RNA Polymerase III Subunit RPC7α (POLR3G) in Cancer. | Cheng, R., et al. 2023. Cancers (Basel). 15: PMID: 37894362
- HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III. | Leone, S., et al. 2024. Mol Cell. 84: 687-701.e7. PMID: 38266641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pol III RPC32 Antibody (C32-1) | sc-21754 | 200 µg/ml | $322.00 | |||
Pol III RPC32 Antibody (C32-1): m-IgG Fc BP-HRP Bundle | sc-525504 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pol III RPC32 Antibody (C32-1) AC | sc-21754 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pol III RPC32 Antibody (C32-1) HRP | sc-21754 HRP | 200 µg/ml | $322.00 | |||
Pol III RPC32 Antibody (C32-1) FITC | sc-21754 FITC | 200 µg/ml | $336.00 | |||
Pol III RPC32 Antibody (C32-1) PE | sc-21754 PE | 200 µg/ml | $349.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 488 | sc-21754 AF488 | 200 µg/ml | $364.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 546 | sc-21754 AF546 | 200 µg/ml | $364.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 594 | sc-21754 AF594 | 200 µg/ml | $364.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 647 | sc-21754 AF647 | 200 µg/ml | $364.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 680 | sc-21754 AF680 | 200 µg/ml | $364.00 | |||
Pol III RPC32 Antibody (C32-1) Alexa Fluor® 790 | sc-21754 AF790 | 200 µg/ml | $364.00 |