Podocin Antibody (G-5): sc-518088

Podocin Antibody (G-5) is a mouse monoclonal IgM antibody that detects Podocin protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Podocin (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Podocin plays a crucial role in maintaining the integrity of the glomerular filtration barrier, which is essential for proper kidney function. Podocin is primarily located in the podocytes of the kidney, where Podocin contributes to the formation of the slit diaphragm, a structure that regulates the passage of proteins and other molecules from the blood into the urine. Disruption of Podocin function can lead to severe kidney disorders, such as autosomal recessive steroid-resistant nephrotic syndrome (SRN1), characterized by significant proteinuria and rapid progression to end-stage renal disease. The human NPHS2 gene, which encodes Podocin, is located on chromosome 1q25-q31 and produces a 383 amino acid protein that exhibits a unique hairpin-like structure, with both amino- and carboxy-termini situated intracellularly. This structural configuration, along with homology to stomatin family proteins, suggests that Podocin may form high-order oligomers that serve a scaffolding function, thereby influencing the stability and functionality of the glomerular filtration barrier. Understanding Podocin′s role in kidney health is vital for developing targeted therapies for related diseases.

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Podocin Antibody (G-5) References:

1. NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome.  |  Boute, N., et al. 2000. Nat Genet. 24: 349-54. PMID: 10742096
2. Interaction with podocin facilitates nephrin signaling.  |  Huber, TB., et al. 2001. J Biol Chem. 276: 41543-6. PMID: 11562357
3. Prevalence, genetics, and clinical features of patients carrying podocin mutations in steroid-resistant nonfamilial focal segmental glomerulosclerosis.  |  Caridi, G., et al. 2001. J Am Soc Nephrol. 12: 2742-2746. PMID: 11729243
4. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin.  |  Schwarz, K., et al. 2001. J Clin Invest. 108: 1621-9. PMID: 11733557
5. Podocin localizes in the kidney to the slit diaphragm area.  |  Roselli, S., et al. 2002. Am J Pathol. 160: 131-9. PMID: 11786407
6. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression.  |  Saleem, MA., et al. 2002. J Am Soc Nephrol. 13: 630-638. PMID: 11856766
7. [Characterization of the NPH2 gene, coding for the glomerular protein podocin, implicated in a familial form of cortico-resistant nephrotic syndrome transmitted as an autosomal recessive].  |  Boute, N., et al. 2002. Nephrologie. 23: 35-6. PMID: 11908478
8. Pinpointing Podocin Trafficking Defects in Kidney Organoids.  |  Tian, P. and Lennon, R. 2023. J Am Soc Nephrol. 34: 2-4. PMID: 36719143
9. Deletion of protein tyrosine phosphatase SHP-1 restores SUMOylation of podocin and reverses the progression of diabetic kidney disease.  |  Lizotte, F., et al. 2023. Kidney Int. 104: 787-802. PMID: 37507049
10. A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome.  |  Kuzmuk, V., et al. 2024. Kidney Int. 105: 744-758. PMID: 37995908
11. In vivo characterization of a podocyte-expressed short podocin isoform.  |  Butt, L., et al. 2023. BMC Nephrol. 24: 378. PMID: 38114895
12. Ibrutinib-associated focal segmental glomerulosclerosis and the impact of podocin mutations in chronic lymphocytic leukemia.  |  Czogalla, J., et al. 2024. Kidney Int. 105: 877-881. PMID: 38336226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Podocin Antibody (G-5) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Podocin Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Podocin Antibody (G-5) Specifications

Alternate Product Name: NPHS2

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 34-55

Epitope Target: Podocin

Epitope Species: mouse

Product Reactivity/Detection: Podocin

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Podocin Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin