PNPase Antibody (C-5): sc-271973
- PNPase Antibody (C-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 85-117 near the N-terminus of PNPase of human origin
- recommended for detection of PNPase of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for PNPase Antibody (C-5) for WB applications. This reagent is now offered in a bundle with PNPase Antibody (C-5) (see ordering information below).
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SEE ALSO...
PNPase Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects PNPase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PNPase, also known as mitochondrial polyribonucleotide nucleotidyltransferase, 3′-5′ RNA exonuclease, OLD35, or PNPT1, plays a crucial role in mRNA degradation by hydrolyzing single-stranded ribonucleotides in the 3′ to 5′ direction, which is essential for maintaining cellular RNA homeostasis and regulating gene expression. PNPase is primarily located in the mitochondria, where PNPase forms homotrimers and is upregulated in response to interferon-β induction, highlighting PNPase′s involvement in cellular stress responses. The N-terminus of PNPase contains a putative mitochondrial targeting sequence, and mutation analysis has confirmed that these sequences are critical for directing PNPase to the mitochondria, ensuring proper localization and function. Additionally, endogenous PNPase has been shown to co-localize with mitochondrial marker proteins in HeLa cells, further emphasizing PNPase′s importance in mitochondrial RNA metabolism and overall cellular health.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNPase Antibody (C-5) | sc-271973 | 200 µg/ml | $322.00 | |||
PNPase Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-540273 | 200 µg Ab; 10 µg BP | $361.00 | |||
PNPase (C-5) Neutralizing Peptide | sc-271973 P | 100 µg/0.5 ml | $69.00 |