PNPase Antibody (D-1): sc-271479

PNPase Antibody (D-1) is a mouse monoclonal IgG1 antibody that detects PNPase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PNPase antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PNPase, also known as mitochondrial polyribonucleotide nucleotidyltransferase, 3′-5′ RNA exonuclease, OLD35, or PNPT1, plays a crucial role in mRNA degradation by hydrolyzing single-stranded ribonucleotides in the 3′ to 5′ direction, which is essential for regulating gene expression and maintaining cellular RNA homeostasis. PNPase is evolutionarily conserved, with the mouse variant sharing 90% identity with its human counterpart, and forms homotrimers that are upregulated in response to interferon-β induction. The N-terminus of PNPase contains a putative mitochondrial targeting sequence, which has been confirmed through mutation analysis to direct PNPase to the mitochondria, where PNPase co-localizes with mitochondrial marker proteins in HeLa cells. This localization is vital for PNPase function in mitochondrial RNA processing and degradation, highlighting PNPase importance in cellular metabolism and the maintenance of mitochondrial integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PNPase Antibody (D-1) References:

1. Participation of 3'-to-5' exoribonucleases in the turnover of Bacillus subtilis mRNA.  |  Oussenko, IA., et al. 2005. J Bacteriol. 187: 2758-67. PMID: 15805522
2. RNR1, a 3'-5' exoribonuclease belonging to the RNR superfamily, catalyzes 3' maturation of chloroplast ribosomal RNAs in Arabidopsis thaliana.  |  Bollenbach, TJ., et al. 2005. Nucleic Acids Res. 33: 2751-63. PMID: 15891117
3. Conserved domains in polynucleotide phosphorylase among eubacteria.  |  Bermúdez-Cruz, RM., et al. 2005. Biochimie. 87: 737-45. PMID: 16054527
4. Defining the domains of human polynucleotide phosphorylase (hPNPaseOLD-35) mediating cellular senescence.  |  Sarkar, D., et al. 2005. Mol Cell Biol. 25: 7333-43. PMID: 16055741
5. Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.  |  Gewartowski, K., et al. 2006. Acta Biochim Pol. 53: 179-88. PMID: 16505900
6. Human polynucleotide phosphorylase: location matters.  |  Chen, HW., et al. 2007. Trends Cell Biol. 17: 600-8. PMID: 17983748
7. Analysis of the human polynucleotide phosphorylase (PNPase) reveals differences in RNA binding and response to phosphate compared to its bacterial and chloroplast counterparts.  |  Portnoy, V., et al. 2008. RNA. 14: 297-309. PMID: 18083836
8. Stable PNPase RNAi silencing: its effect on the processing and adenylation of human mitochondrial RNA.  |  Slomovic, S. and Schuster, G. 2008. RNA. 14: 310-23. PMID: 18083837

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PNPase Antibody (D-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PNPase Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PNPase Antibody (D-1) Specifications

Alternate Product Name: PNPase

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 656-779

Epitope Target: PNPase

Epitope Species: human

Product Reactivity/Detection: PNPase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PNPase Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin