PMCA1/4 Antibody (5F10): sc-20028
- PMCA1/4 Antibody (5F10) is a mouse monoclonal IgG2a κ PMCA1/4 antibody, cited in 14 publications, provided at 200 µg/ml
- epitope mapping to amino acids 719-738 of PMCA1/4 of human origin
- PMCA1/4 Antibody (5F10) is recommended for detection of PMCA1b, PMCA4a and PMCA4b of mouse, rat, human and Arabadopsis origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine, feline and canine
- Anti-PMCA1/4 Antibody (5F10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PMCA1/4 Antibody (5F10). This work is in progress.
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PMCA1/4 Antibody (5F10) is a mouse monoclonal IgG2a kappa light chain antibody that detects PMCA1/4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PMCA1/4 antibody (5F10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The plasma membrane Ca2+-pumping ATPase (PMCA) family consists of four genes, PMCA1-4, with PMCA4b being predominantly expressed in developing mammary tissue. During lactation, PMCA1b expression increases while PMCA4b expression decreases, highlighting the critical role of PMCAs in maintaining cellular calcium homeostasis. Notably, PMCA4b undergoes cleavage at Asp 1080 by caspase-3, resulting in a fully active fragment that responds more rapidly to increases in calcium levels compared to its autoinhibited form. This rapid response is essential for regulating intracellular calcium levels, particularly in apoptotic cells, where PMCA4b may play a significant role. Additionally, PMCA1 and PMCA4 are expressed at comparable levels in astrocytes and neurons, indicating their importance in neuronal function and signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PMCA1/4 Antibody (5F10) References:
- Ca2+-ATPases and their expression in the mammary gland of pregnant and lactating rats. | Reinhardt, TA. and Horst, RL. 1999. Am J Physiol. 276: C796-802. PMID: 10199809
- Plasma membrane calcium ATPase isoforms in astrocytes. | Fresu, L., et al. 1999. Glia. 28: 150-5. PMID: 10533058
- Ca(2+)-ATPase protein expression in mammary tissue. | Reinhardt, TA., et al. 2000. Am J Physiol Cell Physiol. 279: C1595-602. PMID: 11029307
- Plasma membrane Ca2+ATPase isoform 4b is cleaved and activated by caspase-3 during the early phase of apoptosis. | Pászty, K., et al. 2002. J Biol Chem. 277: 6822-9. PMID: 11751908
- Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels. | Brandt, P., et al. 1992. J Biol Chem. 267: 4376-85. PMID: 1531651
- Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules. | Borke, JL., et al. 1989. Am J Physiol. 257: F842-9. PMID: 2556040
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMCA1/4 Antibody (5F10) | sc-20028 | 200 µg/ml | $316.00 | |||
PMCA1/4 Antibody (5F10) AC | sc-20028 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PMCA1/4 Antibody (5F10) HRP | sc-20028 HRP | 200 µg/ml | $316.00 | |||
PMCA1/4 Antibody (5F10) FITC | sc-20028 FITC | 200 µg/ml | $330.00 | |||
PMCA1/4 Antibody (5F10) PE | sc-20028 PE | 200 µg/ml | $343.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 488 | sc-20028 AF488 | 200 µg/ml | $357.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 546 | sc-20028 AF546 | 200 µg/ml | $357.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 594 | sc-20028 AF594 | 200 µg/ml | $357.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 647 | sc-20028 AF647 | 200 µg/ml | $357.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 680 | sc-20028 AF680 | 200 µg/ml | $357.00 | |||
PMCA1/4 Antibody (5F10) Alexa Fluor® 790 | sc-20028 AF790 | 200 µg/ml | $357.00 |