PMCA Antibody (H-8): sc-271194

PMCA Antibody (H-8) is a mouse monoclonal IgG2a antibody that detects plasma membrane-type Ca2+-ATPases (PMCAs) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PMCA antibody (H-8) plays a crucial role in understanding the function of PMCAs, which are essential for maintaining calcium homeostasis in eukaryotic cells by mediating the export of bivalent calcium ions. PMCAs are vital for numerous cellular processes, including muscle contraction, neurotransmitter release, and cell signaling, as these proteins help regulate intracellular calcium levels. PMCAs adapt to the physiological needs of different tissues and cell types, with expression being tightly regulated during development and in response to various stimuli. PMCA monoclonal antibody (H-8) enables researchers to gain insights into specific isoforms and splice variants of PMCAs active in different cellular contexts, thereby enhancing understanding of calcium signaling pathways and their implications in health and disease.

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PMCA Antibody (H-8) References:

1. Plasma membrane calcium pump (PMCA) differential exposure of hydrophobic domains after calmodulin and phosphatidic acid activation.  |  Mangialavori, I., et al. 2011. J Biol Chem. 286: 18397-404. PMID: 21454645
2. SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells.  |  Philippe, R., et al. 2015. Biochim Biophys Acta. 1853: 892-903. PMID: 25661196
3. Inhibition of PMCA activity by tau as a function of aging and Alzheimer's neuropathology.  |  Berrocal, M., et al. 2015. Biochim Biophys Acta. 1852: 1465-76. PMID: 25892185
4. Phospholipids and calmodulin modulate the inhibition of PMCA activity by tau.  |  Berrocal, M., et al. 2017. Biochim Biophys Acta Mol Cell Res. 1864: 1028-1035. PMID: 27818274
5. Methylene blue activates the PMCA activity and cross-interacts with amyloid β-peptide, blocking Aβ-mediated PMCA inhibition.  |  Berrocal, M., et al. 2018. Neuropharmacology. 139: 163-172. PMID: 30003902
6. Application of PMCA to screen for prion infection in a human cell line used to produce biological therapeutics.  |  Lyon, A., et al. 2019. Sci Rep. 9: 4847. PMID: 30890734
7. PMCA-generated prions from the olfactory mucosa of patients with Fatal Familial Insomnia cause prion disease in mice.  |  Bistaffa, E., et al. 2021. Elife. 10: PMID: 33851575
8. Sorcin Activates the Brain PMCA and Blocks the Inhibitory Effects of Molecular Markers of Alzheimer's Disease on the Pump Activity.  |  Berrocal, M., et al. 2021. Int J Mol Sci. 22: PMID: 34205207
9. Impaired regulation of PMCA activity by defective CFTR expression promotes epithelial cell damage in alcoholic pancreatitis and hepatitis.  |  Madácsy, T., et al. 2022. Cell Mol Life Sci. 79: 265. PMID: 35484438
10. The PLEKHA7-PDZD11 complex regulates the localization of the calcium pump PMCA and calcium handling in cultured cells.  |  Sluysmans, S., et al. 2022. J Biol Chem. 298: 102138. PMID: 35714771

The preferred PMCA antibody is PMCA Antibody (F-3): sc-271917.