SAK/STK18/PLK4 Antibody (D-6): sc-518222

PLK4 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PLK4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PLK4 Antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PLK4, a member of the polo-like kinase family, plays a crucial role in regulating cell cycle progression, particularly during mitosis. PLK4′s unique structure, characterized by a single polo box that forms a dimer fold, is essential for localization to the nucleolus, centrosomes, and cleavage furrow, which are critical sites for cell division. PLK4′s proper functioning is vital for late mitotic progression and cell survival; any dysregulation can lead to increased apoptosis and anaphase arrest. Haploinsufficiency of PLK4 has been linked to the spontaneous development of tumors, particularly in the liver, highlighting PLK4′s importance in maintaining cellular integrity and preventing oncogenesis. With diverse applications and significant role in cell biology, anti-PLK4 antibody (D-6) serves as an invaluable tool for researchers studying cell proliferation and cancer biology.

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SAK/STK18/PLK4 Antibody (D-6) References:

1. Late mitotic failure in mice lacking Sak, a polo-like kinase.  |  Hudson, JW., et al. 2001. Curr Biol. 11: 441-6. PMID: 11301255
2. Polo-like kinase-2 is required for centriole duplication in mammalian cells.  |  Warnke, S., et al. 2004. Curr Biol. 14: 1200-7. PMID: 15242618
3. Differential regulation of polo-like kinase 1, 2, 3, and 4 gene expression in mammalian cells and tissues.  |  Winkles, JA. and Alberts, GF. 2005. Oncogene. 24: 260-6. PMID: 15640841
4. The Plk3-Cdc25 circuit.  |  Myer, DL., et al. 2005. Oncogene. 24: 299-305. PMID: 15640846
5. Sak/Plk4 and mitotic fidelity.  |  Swallow, CJ., et al. 2005. Oncogene. 24: 306-12. PMID: 15640847
6. SAK, a new polo-like kinase, is transcriptionally repressed by p53 and induces apoptosis upon RNAi silencing.  |  Li, J., et al. 2005. Neoplasia. 7: 312-23. PMID: 15967108
7. Plk4 haploinsufficiency causes mitotic infidelity and carcinogenesis.  |  Ko, MA., et al. 2005. Nat Genet. 37: 883-8. PMID: 16025114
8. The Polo kinase Plk4 functions in centriole duplication.  |  Habedanck, R., et al. 2005. Nat Cell Biol. 7: 1140-6. PMID: 16244668
9. PLK4: a promising target for cancer therapy.  |  Zhao, Y. and Wang, X. 2019. J Cancer Res Clin Oncol. 145: 2413-2422. PMID: 31492983
10. Inhibition of PLK4 remodels histone methylation and activates the immune response via the cGAS-STING pathway in TP53-mutated AML.  |  Man, CH., et al. 2023. Blood. 142: 2002-2015. PMID: 37738460
11. PLK4 self-phosphorylation drives the selection of a single site for procentriole assembly.  |  Scott, P., et al. 2023. J Cell Biol. 222: PMID: 37773039
12. Sak, a murine protein-serine/threonine kinase that is related to the Drosophila polo kinase and involved in cell proliferation.  |  Fode, C., et al. 1994. Proc Natl Acad Sci U S A. 91: 6388-92. PMID: 8022793

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SAK/STK18/PLK4 Antibody (D-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SAK/STK18/PLK4 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SAK/STK18/PLK4 Antibody (D-6) Specifications

Alternate Product Name: PLK4

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-264

Epitope Target: PLK4

Epitope Species: human

Product Reactivity/Detection: PLK4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SAK/STK18/PLK4 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin