Plk Antibody (F-8): sc-17783

Plk Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Plk of mouse, rat, and human origin by WB, IP, IF, and ELISA. Plk Antibody (F-8) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Plk, or polo-like kinase 1, is a crucial serine/threonine kinase that plays a significant role in cell cycle regulation, particularly during mitosis. Plk is essential for the proper functioning of the mitotic spindle, which is responsible for the accurate segregation of chromosomes during cell division. Plk is predominantly located in the cytoplasm and accumulates during the S and G2 phases of the cell cycle, with levels peaking at the onset of mitosis before rapidly decreasing post-mitosis. This dynamic expression pattern highlights Plk importance in cell proliferation and suggests Plk may serve as a valuable marker for mitotically active tissues, including the colon and placenta, as well as various tumors. Plk Antibody (F-8) enables researchers to gain insights into the mechanisms of cell division and potential implications of Plk in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Plk Antibody (F-8) References:

1. Polo-like kinase (PLK) inhibitors in preclinical and early clinical development in oncology.  |  Schöffski, P. 2009. Oncologist. 14: 559-70. PMID: 19474163
2. Systematic analysis of the Plk-mediated phosphoregulation in eukaryotes.  |  Liu, Z., et al. 2013. Brief Bioinform. 14: 344-60. PMID: 22851512
3. PLK-1 Targeted Inhibitors and Their Potential against Tumorigenesis.  |  Kumar, S. and Kim, J. 2015. Biomed Res Int. 2015: 705745. PMID: 26557691
4. Regulatory functional territory of PLK-1 and their substrates beyond mitosis.  |  Kumar, S., et al. 2017. Oncotarget. 8: 37942-37962. PMID: 28415805
5. polo, a mitotic mutant of Drosophila displaying abnormal spindle poles.  |  Sunkel, CE. and Glover, DM. 1988. J Cell Sci. 89 (Pt 1): 25-38. PMID: 3417791
6. PLK inhibitors come of age in pediatric brain tumors.  |  Maegawa, S. and Gopalakrishnan, V. 2022. Neuro Oncol. 24: 427-428. PMID: 35015893
7. Cell cycle- and terminal differentiation-associated regulation of the mouse mRNA encoding a conserved mitotic protein kinase.  |  Lake, RJ. and Jelinek, WR. 1993. Mol Cell Biol. 13: 7793-801. PMID: 7902533
8. Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5.  |  Golsteyn, RM., et al. 1994. J Cell Sci. 107 (Pt 6): 1509-17. PMID: 7962193
9. Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase.  |  Hamanaka, R., et al. 1994. Cell Growth Differ. 5: 249-57. PMID: 8018557
10. Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors.  |  Holtrich, U., et al. 1994. Proc Natl Acad Sci U S A. 91: 1736-40. PMID: 8127874
11. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5.  |  Kitada, K., et al. 1993. Mol Cell Biol. 13: 4445-57. PMID: 8321244
12. Malignant transformation of mammalian cells initiated by constitutive expression of the polo-like kinase.  |  Smith, MR., et al. 1997. Biochem Biophys Res Commun. 234: 397-405. PMID: 9177283

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Plk Antibody (F-8) has 247 citations in a variety of scientific publications.

Primary antibodies like Anti-Plk Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Plk Antibody (F-8) Specifications

Alternate Product Name: Plk

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 261-412

Epitope Target: Plk

Epitope Species: human

Product Reactivity/Detection: Plk

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 247 citations in various scientific publications

Available Conjugates/Forms for Plk Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin