PLC δ1 Antibody (B-9): sc-376058

PLC δ1 Antibody (B-9) is a mouse monoclonal IgG2b antibody that detects phosphoinositide-specific phospholipase C δ1 (PLC δ1) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PLC δ1 plays a pivotal role in calcium signaling, acting as a calcium signal amplifier that is activated by atypical GTP-binding proteins, thereby facilitating critical receptor-mediated signal transduction pathways. PLC δ1 is predominantly expressed in the brain, heart, lung, and testis, and abnormal PLC δ1 accumulation has been observed in the brains of individuals with Alzheimer′s disease, indicating a potential involvement in the disease′s pathology. The structure of PLC δ1 includes several functional domains that enable calcium binding as a cofactor, which is essential for enzymatic activity in generating the second messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol 4,5-bisphosphate. Understanding the structural characteristics of PLC δ1 is crucial, as these features dictate functional capabilities and interactions with other signaling molecules, thereby influencing various physiological processes. Anti-PLC δ1 antibody (B-9) is an invaluable tool for researchers investigating the intricate signaling pathways mediated by phospholipase C and its implications in health and disease.

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PLC δ1 Antibody (B-9) References:

1. Regulation of phospholipase C-delta1 through direct interactions with the small GTPase Ral and calmodulin.  |  Sidhu, RS., et al. 2005. J Biol Chem. 280: 21933-41. PMID: 15817490
2. Regulation of phospholipase C-delta1 by ARGHAP6, a GTPase-activating protein for RhoA: possible role for enhanced activity of phospholipase C in hypertension.  |  Ochocka, AM., et al. 2008. Int J Biochem Cell Biol. 40: 2264-73. PMID: 18434237
3. Coronary vasospasm induced in transgenic mouse with increased phospholipase C-δ1 activity.  |  Shibutani, S., et al. 2012. Circulation. 125: 1027-36. PMID: 22265909
4. Structural basis for calcium and phosphatidylserine regulation of phospholipase C δ1.  |  Lomasney, JW., et al. 2012. Biochemistry. 51: 2246-57. PMID: 22385159
5. Intramolecular allosteric interaction in the phospholipase C-δ1 pleckstrin homology domain.  |  Tanio, M. and Nishimura, K. 2013. Biochim Biophys Acta. 1834: 1034-43. PMID: 23388389
6. Ca2+-independent binding of anionic phospholipids by phospholipase C δ1 EF-hand domain.  |  Cai, J., et al. 2013. J Biol Chem. 288: 37277-88. PMID: 24235144
7. Critical roles of Gi/o proteins and phospholipase C-δ1 in the activation of receptor-operated TRPC4 channels.  |  Thakur, DP., et al. 2016. Proc Natl Acad Sci U S A. 113: 1092-7. PMID: 26755577
8. Intracellular acidification facilitates receptor-operated TRPC4 activation through PLCδ1 in a Ca2+ -dependent manner.  |  Thakur, DP., et al. 2020. J Physiol. 598: 2651-2667. PMID: 32338378
9. The Pleckstrin Homology Domain of PLCδ1 Exhibits Complex Dissociation Properties at the Inner Leaflet of Plasma Membrane Sheets.  |  Sponholtz, MR. and Senning, EN. 2021. ACS Chem Neurosci. 12: 2072-2078. PMID: 34048227
10. Reduction of platelet phospholipase C-delta1 activity in Alzheimer's disease associated with a specific apolipoprotein E genotype (epsilon3/epsilon3).  |  Matsushima, H., et al. 1998. Int J Mol Med. 1: 91-3. PMID: 9852204

The preferred PLC δ1 antibody is PLC δ1 Antibody (D-7): sc-393464.