Anti-PLC beta 3 Antibody (D-7) recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLC β3 Antibody (D-7): sc-133231

Anti-PLC β3 Antibody (D-7) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-PLC β3 Antibody (D-7) is a mouse monoclonal IgG2a κ PLC β3 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 1151-1234 of PLC β3 of human origin
    • Anti-PLC beta 3 Antibody (D-7) is recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PLC beta 3 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC β3 (D-7): sc-133231.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PLC β3 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC β3 Antibody (D-7) (see ordering information below).
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I am using PLC β3 (D-7): sc-133231 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very efficient PLC β3 antibody works really well in Western Blot, the working dilution is 1: 500,very efficient antibody indeed.
Date published: 2017-02-04
Rated 4 out of 5 by from Publishable data in IP using human epithelial Publishable data in IP using human epithelial cell lines.
Date published: 2015-05-15
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-03-26
Rated 4 out of 5 by from Publishable data in WB using human epithelial Publishable data in WB using human epithelial cell lines. -SCBT Publication Review
Date published: 2015-03-16
Rated 4 out of 5 by from Publishable data in WB using rat hippocampal Publishable data in WB using rat hippocampal neurons. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-02-22
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