PLC β3 Antibody (D-7): sc-133231

PLC β3 Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects PLC beta 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PLC β3 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PLC beta 3 protein plays a vital role in cellular signaling pathways by catalyzing the hydrolysis of phosphatidylinositol 4, 5-bisphosphate into inositol 1, 4, 5-trisphosphate and diacylglycerol, which are crucial second messengers in regulating various physiological processes, including cell growth, differentiation, and apoptosis. PLC beta 3 is predominantly located in the plasma membrane, where PLC beta 3 interacts with G protein-coupled receptors and is activated by Gq class heterotrimeric G proteins, highlighting its importance in mediating receptor signaling. PLC beta 3 presence in various tissues underscores its significance in both normal physiology and pathological conditions, making PLC beta 3 a valuable target for research and therapeutic applications.

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PLC β3 Antibody (D-7) References:

1. Regulation of inositol phospholipid-specific phospholipase C isozymes.  |  Rhee, SG. and Choi, KD. 1992. J Biol Chem. 267: 12393-6. PMID: 1319994
2. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
3. A second gene product of the inositol-phospholipid-specific phospholipase C delta subclass.  |  Meldrum, E., et al. 1991. Eur J Biochem. 196: 159-65. PMID: 1848183
4. Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5.  |  Xiao, W., et al. 2009. Cancer Cell. 16: 161-71. PMID: 19647226
5. Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain.  |  Lyon, AM., et al. 2013. Nat Struct Mol Biol. 20: 355-62. PMID: 23377541
6. M3 muscarinic receptor interaction with phospholipase C β3 determines its signaling efficiency.  |  Kan, W., et al. 2014. J Biol Chem. 289: 11206-11218. PMID: 24596086
7. A second type of rat phosphoinositide-specific phospholipase C containing a src-related sequence not essential for phosphoinositide-hydrolyzing activity.  |  Emori, Y., et al. 1989. J Biol Chem. 264: 21885-90. PMID: 2557343
8. Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.  |  Suh, PG., et al. 1988. Proc Natl Acad Sci U S A. 85: 5419-23. PMID: 2840660
9. Cloning of cDNA encoding rat phospholipase C-beta 4, a new member of the phospholipase C.  |  Kim, MJ., et al. 1993. Biochem Biophys Res Commun. 194: 706-12. PMID: 7688223
10. Activation of phospholipase C beta 2 by the alpha and beta gamma subunits of trimeric GTP-binding protein.  |  Wu, D., et al. 1993. Proc Natl Acad Sci U S A. 90: 5297-301. PMID: 8389480
11. Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3.  |  Jhon, DY., et al. 1993. J Biol Chem. 268: 6654-61. PMID: 8454637
12. Contribution of phospholipase C-beta3 phosphorylation to the rapid attenuation of opioid-activated phosphoinositide response.  |  Strassheim, D., et al. 1998. Mol Pharmacol. 53: 1047-53. PMID: 9614207

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PLC β3 Antibody (D-7) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-PLC β3 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PLC β3 Antibody (D-7) Specifications

Alternate Product Name: PLC β3

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1151-1234

Epitope Target: PLC β3

Epitope Species: human

Product Reactivity/Detection: PLC β3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for PLC β3 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin