PKN Antibody (A-8): sc-7969

PKN Antibody (A-8) is a mouse monoclonal IgG3 antibody that detects PKN in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PKN, or protein kinase N, plays a crucial role in cellular signaling by acting as a serine/threonine kinase that is activated by fatty acids and is essential for various cellular processes, including the regulation of the cytoskeleton and cell division. PKN′s unique structure, which includes a catalytic domain homologous to the protein kinase C family, allows PKN to interact specifically with Rho GTPases, thereby functioning as a molecular switch that modulates actin dynamics and cytokinesis. PKN′s structural importance lies in facilitating the phosphorylation of target proteins, such as the head-rod domain of neurofilament protein, which is vital for maintaining neuronal integrity and function. PKN′s interaction with other proteins, such as rhophilin and Citron, underscores PKN′s role in orchestrating complex signaling pathways that are critical for cellular responses to external stimuli. PKN (A-8) monoclonal antibody is an invaluable tool for researchers aiming to explore the intricate mechanisms of cell signaling and cytoskeletal organization in human cells.

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PKN Antibody (A-8) References:

1. Hyaluronan-CD44 interaction with Rac1-dependent protein kinase N-gamma promotes phospholipase Cgamma1 activation, Ca(2+) signaling, and cortactin-cytoskeleton function leading to keratinocyte adhesion and differentiation.  |  Bourguignon, LY., et al. 2004. J Biol Chem. 279: 29654-69. PMID: 15123640
2. A selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H-89), inhibits phosphatidylcholine biosynthesis in HeLa cells.  |  Geilen, CC., et al. 1992. FEBS Lett. 309: 381-4. PMID: 1516714
3. Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.  |  Falk, MD., et al. 2014. Biosci Rep. 34: PMID: 27919031
4. Centrosome- and Golgi-Localized Protein Kinase N-Associated Protein Serves As a Docking Platform for Protein Kinase A Signaling and Microtubule Nucleation in Migrating T-Cells.  |  Ong, ST., et al. 2018. Front Immunol. 9: 397. PMID: 29545805
5. Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.  |  Wallroth, A., et al. 2019. Nat Cell Biol. 21: 1093-1101. PMID: 31451768
6. ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition.  |  Chu, N., et al. 2023. FASEB J. 37: e22779. PMID: 36723798
7. Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion.  |  Yoshida, S., et al. 2024. Nat Commun. 15: 7638. PMID: 39266515
8. Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho.  |  Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
9. Phosphorylation events associated with different states of activation of a hepatic cardiolipin/protease-activated protein kinase. Structural identity to the protein kinase N-type protein kinases.  |  Peng, B., et al. 1996. J Biol Chem. 271: 32233-40. PMID: 8943281
10. The protein kinase N (PKN) gene PRKCL1/Prkcl1 maps to human chromosome 19p12-p13.1 and mouse chromosome 8 with close linkage to the myodystrophy (myd) mutation.  |  Bartsch, JW., et al. 1998. Genomics. 49: 129-32. PMID: 9570957

The preferred PKN antibody is PKN Antibody (H-4): sc-393344.