PKN Antibody (49): sc-136037

PKN Antibody (49) is a mouse monoclonal IgG1 antibody that detects PKN in mouse, rat, human, and canine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PKN, or protein kinase N, plays a crucial role in cellular signaling by acting as a serine/threonine kinase that is activated by fatty acids and is involved in the regulation of various cellular processes, including cytoskeletal dynamics and cell division. PKN′s ability to associate with Rho proteins allows PKN to function as a molecular switch, modulating the actin cytoskeleton and influencing the formation of stress fibers and focal adhesions, which are essential for maintaining cell shape and motility. The unique structure of PKN, which shares homology with the protein kinase C family, enables PKN to phosphorylate specific substrates, such as the head-rod domain of neurofilament protein, thereby impacting neuronal function and stability. PKN′s interaction with other proteins, such as rhophilin and citron, highlights PKN′s importance in orchestrating complex signaling pathways that govern cellular responses to external stimuli. Anti-PKN antibody (49) is an invaluable tool for researchers studying the intricate mechanisms of cell signaling and cytoskeletal organization.

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PKN Antibody (49) References:

1. Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton.  |  Dong, LQ., et al. 2000. Proc Natl Acad Sci U S A. 97: 5089-94. PMID: 10792047
2. Hyperosmotic-induced protein kinase N 1 activation in a vesicular compartment is dependent upon Rac1 and 3-phosphoinositide-dependent kinase 1.  |  Torbett, NE., et al. 2003. J Biol Chem. 278: 32344-51. PMID: 12783890
3. Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.  |  Falk, MD., et al. 2014. Biosci Rep. 34: PMID: 27919031
4. Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.  |  Wallroth, A., et al. 2019. Nat Cell Biol. 21: 1093-1101. PMID: 31451768
5. Protein Kinase N Promotes Stress-Induced Cardiac Dysfunction Through Phosphorylation of Myocardin-Related Transcription Factor A and Disruption of Its Interaction With Actin.  |  Sakaguchi, T., et al. 2019. Circulation. 140: 1737-1752. PMID: 31564129
6. Protein Kinase N Family Negatively Regulates Constitutive Androstane Receptor-Mediated Transcriptional Induction of Cytochrome P450 2b10 in the Livers of Mice.  |  Kawase, A., et al. 2021. J Pharmacol Exp Ther. 379: 53-63. PMID: 34312179
7. ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition.  |  Chu, N., et al. 2023. FASEB J. 37: e22779. PMID: 36723798
8. Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.  |  Wang, D., et al. 2023. Autophagy. 19: 2485-2503. PMID: 36897022
9. Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion.  |  Yoshida, S., et al. 2024. Nat Commun. 15: 7638. PMID: 39266515
10. Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho.  |  Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126

The preferred PKN antibody is PKN Antibody (H-4): sc-393344.