PKM Antibody (C-11): sc-365684

PKM Antibody (C-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects PKM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKM (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKM protein plays a crucial role in cellular metabolism, specifically in glycolysis, where PKM catalyzes the conversion of phosphoenolpyruvate to pyruvate, generating adenosine triphosphate (ATP) in the process. This function is vital for energy production in cells, particularly in tissues with high metabolic demands, such as muscle and brain. PKM gene encodes two isoforms, M1 and M2, through alternative splicing, with both isoforms forming tetramers that are activated by fructose 1,6-bisphosphate. Notably, M2-type isoform has been shown to interact with Oct-4, a key transcription factor in stem cell biology, enhancing transcriptional activity and thereby influencing cellular differentiation and proliferation. This interaction underscores PKM′s importance in not only energy metabolism but also in regulating critical cellular processes, making anti-PKM antibody (C-11) an essential tool for researchers studying metabolic pathways and stem cell dynamics.

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PKM Antibody (C-11) References:

1. Oocyte control of metabolic cooperativity between oocytes and companion granulosa cells: energy metabolism.  |  Sugiura, K., et al. 2005. Dev Biol. 279: 20-30. PMID: 15708555
2. Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis.  |  Dombrauckas, JD., et al. 2005. Biochemistry. 44: 9417-29. PMID: 15996096
3. High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2).  |  Li, Y., et al. 2005. Mol Cell Biochem. 277: 117-25. PMID: 16132722
4. Redox proteomics identification of oxidatively modified hippocampal proteins in mild cognitive impairment: insights into the development of Alzheimer's disease.  |  Butterfield, DA., et al. 2006. Neurobiol Dis. 22: 223-32. PMID: 16466929
5. Identification of pyruvate kinase as an antigen associated with Tourette syndrome.  |  Kansy, JW., et al. 2006. J Neuroimmunol. 181: 165-76. PMID: 17011640
6. Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.  |  Steták, A., et al. 2007. Cancer Res. 67: 1602-8. PMID: 17308100
7. Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription.  |  Lee, J., et al. 2008. Int J Biochem Cell Biol. 40: 1043-54. PMID: 18191611
8. The monomer of pyruvate kinase, subtype M1, is both a kinase and a cytosolic thyroid hormone binding protein.  |  Parkison, C., et al. 1991. Biochem Biophys Res Commun. 179: 668-74. PMID: 1883388
9. In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M2 by glucose is mediated via fructose 1,6-bisphosphate.  |  Ashizawa, K., et al. 1991. J Biol Chem. 266: 16842-6. PMID: 1885610
10. Discovery of Functional Alternatively Spliced PKM Transcripts in Human Cancers.  |  Li, X., et al. 2021. Cancers (Basel). 13: PMID: 33478099
11. Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1.  |  Choksi, A., et al. 2021. Cancer Metab. 9: 16. PMID: 33863392
12. RBX1 regulates PKM alternative splicing to facilitate anaplastic thyroid carcinoma metastasis and aerobic glycolysis by destroying the SMAR1/HDAC6 complex.  |  Xu, D., et al. 2023. Cell Biosci. 13: 36. PMID: 36810109

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKM Antibody (C-11) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-PKM Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKM Antibody (C-11) Specifications

Alternate Product Name: pyruvate kinase M1/2

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 454-513

Epitope Target: PKM

Epitope Species: human

Product Reactivity/Detection: PKM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for PKM Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin