pki γ Antibody (D-8): sc-515108
- pki γ Antibody (D-8) is a mouse monoclonal IgG1 κ pki γ antibody provided at 200 µg/ml
- raised against amino acids 20-76 mapping at the C-terminus of pki γ of human origin
- pki gamma Antibody (D-8) is recommended for detection of pki γ of mouse, rat, human origin by WB, IP, IF and ELISA
- Anti-pki gamma Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pki γ Antibody (D-8) for WB applications. These reagents are now offered in bundles with pki γ Antibody (D-8) (see ordering information below).
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pki γ Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects pki gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). pki γ (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. pki gamma protein, also known as cAMP-dependent protein kinase inhibitor gamma, plays a crucial role in cellular signaling by inhibiting the catalytic subunit of protein kinase A (PKA), which is essential for mediating diverse cellular responses such as proliferation, ion transport, and gene transcription. This inhibition helps regulate the duration and intensity of PKA signaling, thereby influencing processes like metabolism and apoptosis. pki gamma is highly expressed in skeletal muscle, testis, and heart, indicating a significant role in these tissues. Research has shown that knockdown of pki gamma mRNA can enhance the anti-apoptotic effects of parathyroid hormone and β-adrenergic agonists, suggesting that targeting pki gamma may offer therapeutic potential in conditions like osteoporosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
pki γ Antibody (D-8) References:
- Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA. | Zheng, L., et al. 2000. Biochem J. 349: 403-7. PMID: 10880337
- PKA: a portrait of protein kinase dynamics. | Taylor, SS., et al. 2004. Biochim Biophys Acta. 1697: 259-69. PMID: 15023366
- Endogenous protein kinase inhibitor gamma terminates immediate-early gene expression induced by cAMP-dependent protein kinase (PKA) signaling: termination depends on PKA inactivation rather than PKA export from the nucleus. | Chen, X., et al. 2005. J Biol Chem. 280: 2700-7. PMID: 15557275
- Dynamics of signaling by PKA. | Taylor, SS., et al. 2005. Biochim Biophys Acta. 1754: 25-37. PMID: 16214430
- Protein kinase inhibitor peptide (PKI): a family of endogenous neuropeptides that modulate neuronal cAMP-dependent protein kinase function. | Dalton, GD. and Dewey, WL. 2006. Neuropeptides. 40: 23-34. PMID: 16442618
- Endogenous PKI gamma limits the duration of the anti-apoptotic effects of PTH and beta-adrenergic agonists in osteoblasts. | Chen, X., et al. 2007. J Bone Miner Res. 22: 656-64. PMID: 17266398
- Signaling through cAMP and cAMP-dependent protein kinase: diverse strategies for drug design. | Taylor, SS., et al. 2008. Biochim Biophys Acta. 1784: 16-26. PMID: 17996741
- Characterization of PKIgamma, a novel isoform of the protein kinase inhibitor of cAMP-dependent protein kinase. | Collins, SP. and Uhler, MD. 1997. J Biol Chem. 272: 18169-78. PMID: 9218452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pki γ Antibody (D-8) | sc-515108 | 200 µg/ml | $322.00 | |||
pki γ Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-531288 | 200 µg Ab; 10 µg BP | $361.00 | |||
pki γ Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-524938 | 200 µg Ab, 40 µg BP | $361.00 | |||
pki γ Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-544470 | 200 µg Ab; 20 µg BP | $361.00 | |||
pki γ Antibody (D-8) AC | sc-515108 AC | 500 µg/ml, 25% agarose | $424.00 | |||
pki γ Antibody (D-8) HRP | sc-515108 HRP | 200 µg/ml | $322.00 | |||
pki γ Antibody (D-8) FITC | sc-515108 FITC | 200 µg/ml | $336.00 | |||
pki γ Antibody (D-8) PE | sc-515108 PE | 200 µg/ml | $349.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 488 | sc-515108 AF488 | 200 µg/ml | $364.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 546 | sc-515108 AF546 | 200 µg/ml | $364.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 594 | sc-515108 AF594 | 200 µg/ml | $364.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 647 | sc-515108 AF647 | 200 µg/ml | $364.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 680 | sc-515108 AF680 | 200 µg/ml | $364.00 | |||
pki γ Antibody (D-8) Alexa Fluor® 790 | sc-515108 AF790 | 200 µg/ml | $364.00 |