PKC zeta Antibody (H-1): sc-17781

PKC ζ Antibody (H-1) is a mouse monoclonal IgG2a antibody that targets an epitope mapping between amino acids 573-592 at the C-terminus of rat-origin Protein Kinase C zeta (PKC ζ), also known as PRKCZ or nPKC-ζ. Anti-PKC ζ antibody (H-1) reacts with mouse, rat, and human species and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKC ζ is an atypical member of the protein kinase C family, playing a crucial role in various cellular processes such as cell growth, differentiation, survival, gene expression, and membrane function. Unlike conventional PKCs, PKC ζ activation is independent of calcium and diacylglycerol (DAG), allowing unique function within signaling pathways. PKC ζ is involved in the regulation of NF-κB activation, insulin signaling, cell polarity, and the PI3K/Akt pathway, making PKC ζ significant in understanding diseases like diabetes, cancer, and immune disorders. PKC ζ′s involvement in cell survival and proliferation underscores PKC ζ′s importance as a potential therapeutic target. Furthermore, distinct activation mechanisms make PKC ζ an intriguing subject in signal transduction research, potentially revealing new insights into cellular communication pathways. Anti-PKC ζ antibody (H-1) enables researchers to effectively study PKC ζ expression and function in various biological contexts, aiding in the development of novel treatments.

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PKC zeta Antibody (H-1) References:

1. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
2. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC.  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
3. Expression and characterization of protein kinase C-delta.  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
4. PKC isoenzymes in equine platelets and stimulus induced activation.  |  Aktan, I., et al. 2011. Vet Immunol Immunopathol. 141: 276-82. PMID: 21513989
5. PKC-ζ Aggravates Doxorubicin-Induced Cardiotoxicity by Inhibiting Wnt/β-Catenin Signaling.  |  Cao, YJ., et al. 2022. Front Pharmacol. 13: 798436. PMID: 35237161
6. PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer.  |  Zhang, F., et al. 2023. Breast Cancer Res. 25: 38. PMID: 37029374
7. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
8. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
9. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
10. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
11. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
12. Intrarenal distribution of rabbit PKC zeta.  |  Hao, CM., et al. 1997. Kidney Int. 51: 1831-7. PMID: 9186872

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKC zeta Antibody (H-1) has 146 citations in a variety of scientific publications.

Primary antibodies like Anti-PKC zeta Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKC zeta Antibody (H-1) Specifications

Alternate Product Name: PKC zeta

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 573-592

Epitope Target: PKC ζ

Epitope Species: rat

Product Reactivity/Detection: PKC ζ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 146 citations in various scientific publications

Available Conjugates/Forms for PKC zeta Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-PKC zeta antibody is p-PKC zeta Antibody (H-2): sc-271962.