p-PKC zeta Antibody (H-2): sc-271962

p-PKC ζ Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thr 410 phosphorylated PKC ζ from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-PKC ζ (H-2) antibody is available in non-conjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The protein kinase C (PKC) family, to which PKC ζ belongs, regulates numerous cellular processes, such as cell growth, differentiation, and apoptosis. PKC ζ plays a vital role in mediating signaling pathways that influence cell survival and proliferation, making PKC ζ a significant target for understanding cancer biology and therapeutic interventions. PKC ζ primarily resides in the cytoplasm and translocates to the membrane upon activation, which is essential for signal transduction. PKC ζ interactions with various substrates and signaling molecules underscore PKC ζ importance in cellular communication and response to external stimuli. Phosphorylation of PKC ζ by PDK1 serves as a key regulatory mechanism that activates kinase activity, enabling modulation of downstream signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-PKC zeta Antibody (H-2) References:

1. Protein kinase C delta associates with and phosphorylates Stat3 in an interleukin-6-dependent manner.  |  Jain, N., et al. 1999. J Biol Chem. 274: 24392-400. PMID: 10446219
2. Regulation of Jak2 tyrosine kinase by protein kinase C during macrophage differentiation of IL-3-dependent myeloid progenitor cells.  |  Kovanen, PE., et al. 2000. Blood. 95: 1626-32. PMID: 10688817
3. Thiazolidinedione treatment enhances insulin effects on protein kinase C-zeta /lambda activation and glucose transport in adipocytes of nondiabetic and Goto-Kakizaki type II diabetic rats.  |  Kanoh, Y., et al. 2000. J Biol Chem. 275: 16690-6. PMID: 10749857
4. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
5. [The retinal nerve fiber layer in normal eyes and in glaucoma. I. Semiquantitative data of 398 eyes with glaucoma].  |  Jonas, JB., et al. 1989. Klin Monbl Augenheilkd. 194: 437-46. PMID: 2761192
6. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
7. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
8. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
9. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
10. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKC zeta Antibody (H-2) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKC zeta Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKC zeta Antibody (H-2) Specifications

Alternate Product Name: PKC zeta

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 410

Epitope Target: PKC ζ

Epitope Species: human

Product Reactivity/Detection: Thr 410 phosphorylated PKC ζ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for p-PKC zeta Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PKC zeta antibody is PKC zeta Antibody (H-1): sc-17781.